Citometry Area

Analyzers

CytoFLEX LX - Flow Cytometer

cytoflex_lx.jpeg The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously.

Digital signal processing on 7 decades of dynamic range.

Excellent resolution of nanoparticles thanks to the new VSSC parameter.



Technical specifications

  • Resolution:
    • < 300 nm particles (488 nm side scatter)
    • < 80 nm polystyrene particles (405 nm side scatter)
    • rCV 3%
  • collections vessels:
    • 5 ml, 2 ml, 1.5 ml;
    • 96w plates;
  • absolute counting.


Optical configuration

5 activated lasers / 19 color parameters:

UV Laser
355 nm 		Violet Laser
405 nm 		Blue Laser
488 nm 		Yellow-Green Laser
561 nm 		Red Laser
640 nm
405/30
525/40
675/30 		450/45
525/40
610/20
660/10
763/43 		488/8 SSC
525/40
610/20
690/50 		585/42
610/20
675/30
710/50
763/43 		660/10
712/25
763/43




LSR II - BD Biosciences

Technical features (brochure):

  • 3 laser (8 fluorescence)
    • 405 nm, 2 PMT
    • 488 nm, 4 PMT
    • 633 nm, 2 PMT
  • Sample acquisition possible also from multiwell plates.




FACSCanto - BD Biosciences



Technical features (brochure):

  • 2 laser (6 fluorescence)
    • 488 nm, 4 PMT
    • 633 nm, 2 PMT







GALLIOS - Beckman Coulter

Technical features (brochure, specifications):

  • 3 laser (10 fluorescence)
    • 405 nm, 2 PMT
    • 488 nm, 5 PMT
    • 633 nm, 3 PMT

Rules for users accredited to the Gallios flow cytometer