Citometry Area

Cell Sorter

MoFlo Astrios Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scattering parameters (1 side scattering detectors and 2 forward scattering detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system.

Technical specifications

  • jet-in-air system
  • temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.)
  • available nozzle sizes: 70um, 100um
  • up to 6 populations can be sorted at the same time (only available for 5 mL, 2 mL or 1.5 mL tubes)
  • various collection vessels can be used:
    • 5 mL, 2 mL, 1.5 mL tubes (6-way sort possible)
    • 15 mL tubes (2-way sort)
    • 50 mL tubes (2-way sort)
    • slides
    • 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort)
    • PCR tubes/strips/plates (1-way sort)
  • sort setup modes:
    • purity
    • single cell (cloning)
    • enrichment


Optical Configuration

Violet Laser
405 nm
Blue Laser
488 nm
Yellow Laser
561 nm
Red Laser
640 nm
448/59
546/20
620/29
671/30
488/6
513/26
664/22
710/45
579/19
614/20
692/75
795/70
671/30
722/44
795/70


FACSARIA BD Biosciences Technical features (brochure):

  • 3 laser (9 fluorescence)
    • 405 nm, 2 PMT
    • 488 nm, 5 PMT
    • 633 nm, 2 PMT
  • 2/4 ways of sorting
  • Deposition on multiwell plates and slide