Proteomics Area

MassSpec

Isolation and characterization of exosomes derived from fertile sheep hydatid cysts.
Siles-Lucas M, Sánchez-Ovejero C, González-Sánchez M, González E, Falcón-Pérez JM, Boufana B, Fratini F, Casulli A, Manzano-Román R.
Vet Parasitol. 2017 Mar 15;236:22-33. doi: 10.1016/j.vetpar.2017.01.022. Epub 2017 Jan 23.

Proteomic analysis of detergent-resistant membrane microdomains in trophozoite blood stage of the human malaria parasite Plasmodium falciparum.
Yam XY, Birago C, Fratini F, Di Girolamo F, Raggi C, Sargiacomo M, Bachi A, Berry L, Fall G, Currà C, Pizzi E, Breton CB, Ponzi M.
Mol Cell Proteomics. 2013 Dec;12(12):3948-61. doi: 10.1074/mcp.M113.029272.

Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle.
Possenti A*, Fratini F*, Fantozzi L, Pozio E, Dubey JP, Ponzi M, Pizzi E, Spano F.
BMC Genomics. 2013 Mar 15;14:183. doi: 10.1186/1471-2164-14-183.

Increased levels of acute-phase inflammatory proteins in plasma of patients with sporadic CJD.
Fratini F, Principe S, Puopolo M, Ladogana A, Poleggi A, Piscopo P, Bruno G, Castrechini S, Pascone R, Confaloni A, Minghetti L, Cardone F, Pocchiari M, Crescenzi M.
Neurology. 2012 Sep 4;79(10):1012-8. doi: 10.1212/WNL.0b013e318265a55d.

Serum low-molecular-weight protein fractionation for biomarker discovery.
VanMeter AJ, Camerini S, Polci ML, Tessitore A, Trivedi N, Heiby M, Kamal Y, Hansen J, Zhou W.
Methods Mol Biol. 2012;823:237-49. doi: 10.1007/978-1-60327-216-2_15.

An Integrated Approach to Explore Composition and Dynamics of Cholesterol-rich Membrane Microdomains in Sexual Stages of Malaria Parasite.
Fratini F, Raggi C, Sferra G, Birago C, Sansone A, Grasso F, Curr C, Olivieri A, Pace T, Mochi S, Picci L, Ferreri C, Di Biase A, Pizzi E, Ponzi M.
Mol Cell Proteomics. 2017 Oct;16(10):1801-1814. doi: 10.1074/mcp.M117.067041. Epub 2017 Aug 10.

Mass spectrometry detection of fraudulent use of cow whey in water buffalo, sheep, or goat Italian ricotta cheese.
Camerini S, Montepeloso E, Casella M, Crescenzi M, Marianella RM, Fuselli F.
Food Chem. 2016 Apr 15;197 Pt B:1240-8. doi: 10.1016/j.foodchem.2015.11.073.

Anti-GAPDH Autoantibodies as a Pathogenic Determinant and Potential Biomarker of Neuropsychiatric Diseases.
Delunardo F, Soldati D, Bellisario V, Berry A, Camerini S, Crescenzi M, Alessandri C, Conti F, Ceccarelli F, Francia A, Valesini G, Cirulli F, Siracusano A, Siracusano A, Niolu C, Alex Rubino I, Ortona E, Margutti P.
Arthritis Rheumatol. 2016 Nov;68(11):2708-2716. doi: 10.1002/art.39750.

Autoantibodies specific to D4GDI modulate Rho GTPase mediated cytoskeleton remodeling and induce autophagy in T lymphocytes.
Barbati C, Alessandri C, Vomero M, Vona R, Colasanti T, Vacirca D, Camerini S, Crescenzi M, Pendolino M, Truglia S, Conti F, Garofalo T, Sorice M, Pierdominici M, Valesini G, Malorni W, Ortona E.
See comment in PubMed Commons belowJ Autoimmun. 2015 Apr;58:78-89. doi: 10.1016/j.jaut.2015.01.005. Epub 2015 Jan 24.

Streptococcal-vimentin cross-reactive antibodies induce microvascular cardiac endothelial proinflammatory phenotype in rheumatic heart disease.
Delunardo F, Scalzi V, Capozzi A, Camerini S, Misasi R, Pierdominici M, Pendolino M, Crescenzi M, Sorice M, Valesini G, Ortona E, Alessandri C.
Clin Exp Immunol. 2013 Sep;173(3):419-29. doi: 10.1111/cei.12135.

An insight into the abundant proteome of 46BR.1G1 fibroblasts deficient of DNA ligase I.
Giuliano S, Iadarola P, Leva V, Montecucco A, Camerini S, Crescenzi M, Salvini R, Bardoni A.
Electrophoresis. 2012 Jan;33(2):307-15. doi: 10.1002/elps.201100332.

Phosphorylation of SRSF1 is modulated by replicational stress.
Leva V, Giuliano S, Bardoni A, Camerini S, Crescenzi M, Lisa A, Biamonti G, Montecucco A.
Nucleic Acids Res. 2012 Feb;40(3):1106-17. doi: 10.1093/nar/gkr837.

The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence.
Burla R, Carcuro M, Torre ML, Fratini F, Crescenzi M, D'Apice MR, Spitalieri P, Raffa GD, Astrologo L, Lattanzi G, Cundari E, Raimondo D, Biroccio A, Gatti M, Saggio I.
Open Biol. 2016 Aug;6(8). pii: 160103. doi: 10.1098/rsob.160103.

Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts.
Emanuele M, Esposito A, Camerini S, Antonucci F, Ferrara S, Seghezza S, Catelani T, Crescenzi M, Marotta R, Canale C, Matteoli M, Menna E, Chieregatti E.
EBioMedicine. 2016 May;7:191-204. doi: 10.1016/j.ebiom.2016.03.038.

Pur-alpha functionally interacts with FUS carrying ALS-associated mutations.
Di Salvio M, Piccinni V, Gerbino V, Mantoni F, Camerini S, Lenzi J, Rosa A, Chellini L, Loreni F, Carrì MT, Bozzoni I, Cozzolino M, Cestra G.
Cell Death Dis. 2015 Oct 22;6:e1943. doi: 10.1038/cddis.2015.295.

Megalencephalic leukoencephalopathy with subcortical cysts protein-1 modulates endosomal pH and protein trafficking in astrocytes: relevance to MLC disease pathogenesis.
Brignone MS, Lanciotti A, Visentin S, De Nuccio C, Molinari P, Camerini S, Diociaiuti M, Petrini S, Minnone G, Crescenzi M, Laudiero LB, Bertini E, Petrucci TC, Ambrosini E.
Neurobiol Dis. 2014 Jun;66:1-18. doi: 10.1016/j.nbd.2014.02.003.

The nebulin SH3 domain is dispensable for normal skeletal muscle structure but is required for effective active load bearing in mouse.
Yamamoto DL, Vitiello C, Zhang J, Gokhin DS, Castaldi A, Coulis G, Piaser F, Filomena MC, Eggenhuizen PJ, Kunderfranco P, Camerini S, Takano K, Endo T, Crescenzi M, Luther PK, Lieber RL, Chen J, Bang ML.
J Cell Sci. 2013 Dec 1;126(Pt 23):5477-89. doi: 10.1242/jcs.137026.

Interaction network of the 14-3-3 protein in the ancient protozoan parasite Giardia duodenalis.
Lalle M, Camerini S, Cecchetti S, Sayadi A, Crescenzi M, Pozio E.
J Proteome Res. 2012 May 4;11(5):2666-83. doi: 10.1021/pr3000199.

Post translational modifications (PTM)

Src inhibitors modulate frataxin protein levels.
Cherubini F, Serio D, Guccini I, Fortuni S, Arcuri G, Condò I, Rufini A, Moiz S, Camerini S, Crescenzi M, Testi R, Malisan F.
Hum Mol Genet. 2015 Aug 1;24(15):4296-305. doi: 10.1093/hmg/ddv162.

HIPK2 sustains apoptotic response by phosphorylating Che-1/AATF and promoting its degradation.
De Nicola F, Catena V, Rinaldo C, Bruno T, Iezzi S, Sorino C, Desantis A, Camerini S, Crescenzi M, Floridi A, Passananti C, Soddu S, Fanciulli M.
Cell Death Dis. 2014 Sep 11;5:e1414. doi: 10.1038/cddis.2014.381.

The crystal structure of Giardia duodenalis 14-3-3 in the apo form: when protein post-translational modifications make the difference.
Fiorillo A, di Marino D, Bertuccini L, Via A, Pozio E, Camerini S, Ilari A, Lalle M.
PLoS One. 2014 Mar 21;9(3):e92902. doi: 10.1371/journal.pone.0092902.

Interkingdom complementation reveals structural conservation and functional divergence of 14-3-3 proteins.
Lalle M, Leptourgidou F, Camerini S, Pozio E, Skoulakis EM.
PLoS One. 2013 Oct 11;8(10):e78090. doi: 10.1371/journal.pone.0078090.

HIPK2 catalytic activity and subcellular localization are regulated by activation-loop Y354 autophosphorylation.
Siepi F, Gatti V, Camerini S, Crescenzi M, Soddu S.
Biochim Biophys Acta. 2013 Jun;1833(6):1443-53. doi: 10.1016/j.bbamcr.2013.02.018.

Phosphorylation and nitration of tyrosine residues affect functional properties of Synaptophysin and Dynamin I, two proteins involved in exo-endocytosis of synaptic vesicles.
Mallozzi C, D'Amore C, Camerini S, Macchia G, Crescenzi M, Petrucci TC, Di Stasi AM.
Biochim Biophys Acta. 2013 Jan;1833(1):110-21. doi: 10.1016/j.bbamcr.2012.10.022.

Phosphorylation on threonine 11 of β-dystrobrevin alters its interaction with kinesin heavy chain.
Fratini F, Macchia G, Torreri P, Matteucci A, Salzano AM, Crescenzi M, Macioce P, Petrucci TC, Ceccarini M.
FEBS J. 2012 Nov;279(22):4131-44. doi: 10.1111/febs.12006.

Proteomic and functional analyses reveal pleiotropic action of the anti-tumoral compound NBDHEX in Giardia duodenalis.
Camerini S, Bocedi A, Cecchetti S, Casella M, Carbo M, Morea V, Pozio E, Ricci G, Lalle M.
Int J Parasitol Drugs Drug Resist. 2017 Aug;7(2):147-158. doi: 10.1016/j.ijpddr.2017.03.006. Epub 2017 Mar 29.

The FAD-dependent glycerol-3-phosphate dehydrogenase of Giardia duodenalis: an unconventional enzyme that interacts with the g14-3-3 and it is a target of the antitumoral compound NBDHEX.
Lalle M, Camerini S, Cecchetti S, Finelli R, Sferra G, Müller J, Ricci G, Pozio E.
Front Microbiol. 2015 Jun 1;6:544. doi: 10.3389/fmicb.2015.00544.

Carbon monoxide signaling in human red blood cells: evidence for pentose phosphate pathway activation and protein deglutathionylation.
Metere A, Iorio E, Scorza G, Camerini S, Casella M, Crescenzi M, Minetti M, Pietraforte D.
Antioxid Redox Signal. 2014 Jan 20;20(3):403-16. doi: 10.1089/ars.2012.5102.

Gaucher disease due to saposin C deficiency is an inherited lysosomal disease caused by rapidly degraded mutant proteins.
Motta M, Camerini S, Tatti M, Casella M, Torreri P, Crescenzi M, Tartaglia M, Salvioli R.
Hum Mol Genet. 2014 Nov 1;23(21):5814-26. doi: 10.1093/hmg/ddu299.

Genotype-phenotype analysis of S326C OGG1 polymorphism: a risk factor for oxidative pathologies.
Simonelli V, Camerini S, Mazzei F, Van Loon B, Allione A, D'Errico M, Barone F, Minoprio A, Ricceri F, Guarrera S, Russo A, Dalhus B, Crescenzi M, Hübscher U, Bjørås M, Matullo G, Dogliotti E.
Free Radic Biol Med. 2013 Oct;63:401-9. doi: 10.1016/j.freeradbiomed.2013.05.031.

The role of protein and peptide separation before mass spectrometry analysis in clinical proteomics.
Camerini S, Mauri P.
J Chromatogr A. 2015 Feb 13;1381:1-12. doi: 10.1016/j.chroma.2014.12.035. Review.