Old revisions Backlinks Rename Page This is an old revision of the document! Proteomics Area Surface Plasmon Resonance (SPR - Biacore) Abstract Method Applications Abstract The Surface Plasmon Resonance (SPR) technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²). Utilizzando specifici chip, è possibile misurare interazioni di vario genere a seconda della macromolecola immobilizzata sul supporto, indagando così interazioni proteina-proteina, proteina-lipide, proteina-acido nucleico e lipide-lipide. La tecnica ha applicazioni in diversi ambiti, quali Biochimica, Biologia molecolare, Virologia, Tossicologia, Lipidomica, e controllo di qualità di farmaci biotecnologici. According to the specific macromolecule stratified on the chip, it is possible to test mixed interactions such as protein to protein, protein to lipid, lipid to lipid and nucleic acid to protein. Applications span several fields like biochemistry, molecular biology, virology, toxicology, lipidomic as well in GMP. Proteins immobilization Tagged recombinant proteins immobilization Nucleic acid immobilization Lipids immobilization We make use of the Biacore X100 (Cytiva) instrument: https://www.cytivalifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/systems/biacore-x100-p-05730 Contact: Marcello Belfiore ☎ +39 06 4990 3046 Where I am: building 10, floor D, room 9