EPR Area

Surface Plasmon Resonance (SPR - Biacore)

Abstract


The Surface Plasmon Resonance (SPR) technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²).

According to the specific macromolecule stratified on the chip, it is possible to test mixed interactions such as protein to protein, protein to lipid, lipid to lipid and nucleic acid to protein. Applications span several fields like biochemistry, molecular biology, virology, toxicology, lipidomic as well in GMP.


Proteins immobilization


Tagged recombinant proteins immobilization


Nucleic acid immobilization





Lipids immobilization


We make use of the Biacore X100 (Cytiva) instrument: strumento.jpeg

https://www.cytivalifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/systems/biacore-x100-p-05730






To ask for the service:

  1. preliminary contact the Biacore unit staff;
  2. fill out the online form 'Service Request' briefly illustrating the scientific problem.
Contact: Donatella Pietraforte
+39 06 4990 2907
Where I am: building 20, floor D, room 2s