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Proteomics Area
Surface Plasmon Resonance (SPR - Biacore)
Abstract
The Surface Plasmon Resonance (SPR) technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²).
Utilizzando specifici chip, è possibile misurare interazioni di vario genere a seconda della macromolecola immobilizzata sul supporto, indagando così interazioni proteina-proteina, proteina-lipide, proteina-acido nucleico e lipide-lipide. La tecnica ha applicazioni in diversi ambiti, quali Biochimica, Biologia molecolare, Virologia, Tossicologia, Lipidomica, e controllo di qualità di farmaci biotecnologici.
According to the specific macromolecule stratified on the chip, it is possible to test mixed interactions such as protein to protein, protein to lipid, lipid to lipid and nucleic acid to protein. Applications span several fields like biochemistry, molecular biology, virology, toxicology, lipidomic as well in GMP.
Proteins immobilization
Tagged recombinant proteins immobilization
Nucleic acid immobilization
Lipids immobilization
We make use of the Biacore X100 (Cytiva) instrument: 
