Old revisions Backlinks Rename Page Microscopy Area Confocal Microscopy Confocal Laser Scanning Microscopy (CLSM) is a standard technology with the following advantages over conventional widefield microscopy: Removal of light from out-of-focus planes; Improved lateral (XY) and axial (Z) resolution; Ability to perform a series of optical sections from specimens; The confocal microscope is a valuable tool for research because the system is able to obtain images with clarity and reasonable resolution, thus enabling 3-D reconstruction of the specimen from optical sections or series of images obtained. The service involves the use of a latest generation Zeiss LSM980 confocal microscope with Airyscan 2 for high resolution imaging and a Leica TCS SP2 confocal microscope, both equipped with 5 laser sources at different wavelengths. The Unit also provides equipment for sample preparation and maintenance prior to imaging, as well as resources for the data processing. The 3D-imaging properties of the CLSM make it an ideal tool to study cell biology and life sciences. Key applications areas include: localization and co-localization in cells and tissues; cytoskeleton and organelle functions; vesicles trafficking; membrane and ion probes; cell migration and invasion; bioluminescent proteins; epitope tagging; cellular metabolism, oxidative reactions; Cytotoxicity; Stem cell research; In addition, the Unit also provides basic protocols and all reagents needed to perform manually routine histological stainings (e.g. H&E and immune staining). Personnel of the Unit assist scientist throughout the full process of microscopy experiments: design projects, processing data and analyzing images, and interpreting and shaping final results. To ask for the service: preliminary contact the Confocal Microscopy unit staff; fill out the online form 'Service Request' briefly illustrating the scientific problem, type and number of samples, etc.; plan a meeting with the unit staff to discuss methods, costs, timing etc. Contacts: Serena Cecchetti, ☎ +39 06 4990 2966 Francesca Spadaro, ☎ +39 06 4990 6092 Where we are: building 20, floor 2, room 6