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Citometry Area

Cell Sorter

Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scatter parameters (1 side scatter detectors and 2 forward scatter detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system.

Technical specifications

  • Jet-in-air system;
  • Temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.);
  • Available nozzle sizes: 50µm, 70um, 100um;
  • Up to 6 populations can be sorted at the same time (only available for 5mL, 2mL or 1.5mL tubes);
  • Various collection vessels can be used:
    • 5mL, 2mL, 1.5mL tubes (6-way sort possible);
    • 15mL tubes (2-way sort);
    • 50mL tubes (2-way sort);
    • slides;
    • 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort);
    • PCR tubes/strips/plates (1-way sort);
    • sort setup modes: purity, single cell (cloning), enrichment.

FACSARIA BD Biosciences Technical features (brochure):

  • 3 laser (9 fluorescence)
    • 405 nm, 2 PMT
    • 488 nm, 5 PMT
    • 633 nm, 2 PMT
  • 2/4 ways of sorting
  • Deposition on multiwell plates and slide