Citometry Area
Cell Sorter
MoFlo Astrios EQ
Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scattering parameters (1 side scattering detectors and 2 forward scattering detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system.
Technical specifications
- jet-in-air system
- temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.)
- available nozzle sizes: 70um, 100um
- up to 6 populations can be sorted at the same time (only available for 5 mL, 2 mL or 1.5 mL tubes)
- various collection vessels can be used:
- 5 mL, 2 mL, 1.5 mL tubes (6-way sort possible)
- 15 mL tubes (2-way sort)
- 50 mL tubes (2-way sort)
- slides
- 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort)
- PCR tubes/strips/plates (1-way sort)
- sort setup modes:
- purity
- single cell (cloning)
- enrichment
Optical Configuration
Violet Laser 405 nm | Blue Laser 488 nm | Yellow Laser 561 nm | Red Laser 640 nm |
---|---|---|---|
448/59 546/20 620/29 671/30 | 488/6 513/26 664/22 710/45 | 579/19 614/20 692/75 795/70 | 671/30 722/44 795/70 |
FACSAria I - BD Biosciences
Technical features (brochure):
- 3 laser (9 fluorescence)
- 405 nm, 2 PMT
- 488 nm, 5 PMT
- 633 nm, 2 PMT
- 2/4 ways of sorting
- Deposition on multiwell plates and slide