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en:aree:epr:altre_risorse:start [2020/10/30 18:34]
Paola Fattibene
en:aree:epr:altre_risorse:start [2024/04/24 19:24] (current)
Gianluca Frustagli [Surface Plasmon Resonance (SPR - Biacore)] New contact.
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 ====== Other instrumental resources ====== ====== Other instrumental resources ======
  
-====== Immuno-spin trapping ​======+=====Immuno-spin trapping=====
  
 The increase of **"​Oxidant Reactive Species"​ (ROS)** produced under conditions of oxidative stress can induce reversible/​irreversible damage in the target molecules through the formation of radical intermediates centered on the target molecules. However, many of these radicals have a short half-life and their monitoring becomes problematic with both direct EPR and spin trapping technique. The increase of **"​Oxidant Reactive Species"​ (ROS)** produced under conditions of oxidative stress can induce reversible/​irreversible damage in the target molecules through the formation of radical intermediates centered on the target molecules. However, many of these radicals have a short half-life and their monitoring becomes problematic with both direct EPR and spin trapping technique.
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-==== Stimulated Luminescence instrumentation ====+===== Stimulated Luminescence instrumentation ​=====
  
 +\\
 +
 +=== Thermoluminescence reader ===
  
 +{{aree:​epr:​altre_risorse:​lettore_termoluminescenza_harshaw3500-num.jpeg?​300 |Thermoluminescence reader + laboratory oven for thermal treatments}} {{:​aree:​epr:​altre_risorse:​lettore_termoluminescenza_harshaw3500-dett.jpeg?​300 |Detail of thermoluminescence reader}} Thermoluminescence reader Harshaw, mod. 3500 equipped with software WinREMS for data acquisition and analysis. Thermal cycles up to 600°C (with an uncertainty of +/-1%) with different heating rates, including isothermal decays. Laboratory oven specially designed for TL dosimetry. Run by a microprocessor,​ it allows heating of the sample at controlled temperatures. Different heating cycles available by the dedicated software.
  
 \\ \\
-===== Thermoluminescence reader ===== 
-{{aree:​epr:​altre_risorse:​lettore_termoluminescenza_harshaw3500-num.jpeg?​300|Thermoluminescence reader + laboratory oven for thermal treatments}} {{:​aree:​epr:​altre_risorse:​lettore_termoluminescenza_harshaw3500-dett.jpeg?​300|Detail of thermoluminescence reader}} 
-\\ \\ 
-\\ Thermoluminescence reader Harshaw, mod. 3500 equipped with software WinREMS for data acquisition and analysis. Thermal cycles up to 600°C (with an uncertainty of +/-1%) with different heating rates, including isothermal decays. Laboratory oven specially designed for TL dosimetry. Run by a microprocessor,​ it allows heating of the sample at controlled temperatures. Different heating cycles available by the dedicated software. 
 \\ \\
  
-==== Optically stimulated luminescence reader ​====+=== Optically stimulated luminescence reader ===
  
 {{:​aree:​epr:​altre_risorse:​lettore_osl-tl_riso_da-20.jpeg?​300 |OSL/TL reader}} OSL/TL reader Risø DA-20 (DTU Nutech, Danimarca) equipped with a carousel loading up to 48 samples placed on steel plates of about 10mm in diameter and 0.25mm in thickness. Managed by a dedicated software that allows to set OSL and TL measurements in sequence. The optical stimulation system consists of infrared and visible LEDs; currently it allows stimulation with blue light (470 ± 30nm, with a maximum power of 36mW/cm² in the sample position). The heating system for thermal stimulation allows reaching the maximum temperature of 700°C. Different optical filters are available to select the frequency range of the emitted luminescence. Housing for a radioactive source (<​sup>​90</​sup>​Sr/<​sup>​90</​sup>​Y or <​sup>​241</​sup>​Am) available for sample calibration. {{:​aree:​epr:​altre_risorse:​lettore_osl-tl_riso_da-20.jpeg?​300 |OSL/TL reader}} OSL/TL reader Risø DA-20 (DTU Nutech, Danimarca) equipped with a carousel loading up to 48 samples placed on steel plates of about 10mm in diameter and 0.25mm in thickness. Managed by a dedicated software that allows to set OSL and TL measurements in sequence. The optical stimulation system consists of infrared and visible LEDs; currently it allows stimulation with blue light (470 ± 30nm, with a maximum power of 36mW/cm² in the sample position). The heating system for thermal stimulation allows reaching the maximum temperature of 700°C. Different optical filters are available to select the frequency range of the emitted luminescence. Housing for a radioactive source (<​sup>​90</​sup>​Sr/<​sup>​90</​sup>​Y or <​sup>​241</​sup>​Am) available for sample calibration.
  
 +\\
 +\\
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 +=== Pulsed Photostimulated Luminescence (PPSL) ===
  
-==== Pulsed Photostimulated Luminescence ​(PPSL====+{{aree:​epr:​altre_risorse:​lettore_ppsl_suerc.jpeg?​250 |}} {{aree:​epr:​altre_risorse:​lettore_ppsl_suerc-dett.jpeg?​250 |}} Made by the Scottish Universities Environmental Research Centre ​(SUERC, Glasgow Scotlandfor analysis on irradiated foods. The instrument can be connected to a computer and managed by appropriate software. Equipped with a stimulation system of infrared LEDs. Stimulation occurs by pulses: the duration of the single pulse and the number of pulses can be adjusted by the instrument management software.
  
-{{aree:​epr:​altre_risorse:​lettore_ppsl_suerc.jpeg?​250|}} {{aree:​epr:​altre_risorse:​lettore_ppsl_suerc-dett.jpeg?​250|}}+\\
  
-Made by the Scottish Universities Environmental Research Centre (SUERC, Glasgow Scotland) for analysis on irradiated foods. The instrument can be connected to a computer and managed by appropriate software. Equipped with a stimulation system of infrared LEDs. Stimulation occurs by pulses: the duration ​of the single pulse and the number of pulses can be adjusted by the instrument management software.+<wrap tip>​**[[en:aree:​epr:​altre_risorse:​luminescenze|About ​the phenomena ​of Stimulated Luminescence]]**</​wrap>​
  
-\\<wrap tip>​**[[en:​aree:​epr:​altre_risorse:​luminescenze|About the phenomena of Stimulated Luminescence]]**</​wrap>​+\\
  
 > Contacts: > Contacts:
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 +=====Dosimetric systems=====
  
-====== Dosimetric systems ====== +==== Active dosimeters ====
- +
-===== Active dosimeters ====+
- +
-{{aree:​epr:​altre_risorse:​dosimetri_attivi-1.png?​0x150|}} {{aree:​epr:​altre_risorse:​dosimetri_attivi-2.png?​0x150|}}+
  
 +{{aree:​epr:​altre_risorse:​dosimetri_attivi-1.png?​0x150 |}} {{aree:​epr:​altre_risorse:​dosimetri_attivi-2.png?​0x150 |}}
   * Markus ionization chamber type N23343 (PTW)   * Markus ionization chamber type N23343 (PTW)
   * Silicon diode detector for proton beams type DZA192 (Scanditronix)   * Silicon diode detector for proton beams type DZA192 (Scanditronix)
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-===== Passive dosimeters ​=====+==== Passive dosimeters ====
  
-==== Alanine/EPR dosimetry system ​====+=== Alanine/EPR dosimetry system ===
  
-{{:​en:​aree:​epr:​altre_risorse:​alanine.jpg?​300|Alanine/​EPR dosimetry system}}+{{:​en:​aree:​epr:​altre_risorse:​alanine.jpg?​200|Alanine/​EPR dosimetry system}}
  
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-===== Thermoluminescence dosimeters (TLD) =====+=== Thermoluminescence dosimeters (TLD) ===
  
-{{:​en:​aree:​epr:​altre_risorse:​dosimetri_termoluminescenza.jpeg?​300|Thermoluminescent dosimeters}}+{{:​en:​aree:​epr:​altre_risorse:​dosimetri_termoluminescenza.jpeg?​200|Thermoluminescent dosimeters}}
  
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-===== Radiochromic film dosimeters ​=====+=== Radiochromic film dosimeters ===
  
-{{aree:epr:altre_risorse:​dosimetri_film-1.jpeg?​0x150|}} {{aree:​epr:​altre_risorse:​dosimetri_film-2.png?0x150|}}+{{:en:​aree:​epr:​altre_risorse:​dosimetri_a_film.png?350|}}
  
 +\\
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-===== Phantoms ====+==== Phantoms ====
- +
-{{aree:​epr:​altre_risorse:​fantocci-1.png?​0x150|}} {{aree:​epr:​altre_risorse:​fantocci-2.jpeg?​0x150|}}+
  
 +{{:​en:​aree:​epr:​altre_risorse:​fantocci.png?​350 |}}
   * Alderson Rando phantom   * Alderson Rando phantom
   * PMMA phantoms   * PMMA phantoms
   * Water phantoms   * Water phantoms
  
 +\\
 +\\
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 > Contact: [[cinzia.deangelis@iss.it|Cinzia De Angelis]] > Contact: [[cinzia.deangelis@iss.it|Cinzia De Angelis]]
 > ☎ **+39 06 4990 2248** > ☎ **+39 06 4990 2248**
 +
 +\\
 +
 +===== Surface Plasmon Resonance (SPR - Biacore) =====
 +
 +The <wrap em>​Surface Plasmon Resonance (SPR)</​wrap>​ technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²). **[[.:​biacore:​|[...read more]]]**
 +
 +\\
 +
 +> Contact: [[donatella.pietraforte@iss.it|Donatella Pietraforte]]
 +> ☎ **+39 06 4990 2907**
 +> Where I am: building **20**, floor **D**, room **2s**
  
 /* > FIXME **To be revised.**\\ //(remove this paragraph once is finished)// */ /* > FIXME **To be revised.**\\ //(remove this paragraph once is finished)// */