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en:aree:citometria:strumentazione:cell_sorter [2019/06/27 18:01] Gianluca Frustagli |
en:aree:citometria:strumentazione:cell_sorter [2019/07/31 14:41] (current) Gianluca Frustagli |
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| ====== Cell Sorter ====== | ====== Cell Sorter ====== | ||
| - | ===== MoFlo Astrios ===== | + | ===== MoFlo Astrios EQ ===== |
| - | + | ||
| - | Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scatter parameters (1 side scatter detectors and 2 forward scatter detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system. | + | |
| + | {{ :aree:citometria:strumentazione:moflo-astrios.jpeg?300|MoFlo Astrios}} Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scattering parameters (1 side scattering detectors and 2 forward scattering detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system. | ||
| ==== Technical specifications ==== | ==== Technical specifications ==== | ||
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| * jet-in-air system | * jet-in-air system | ||
| * temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.) | * temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.) | ||
| - | * available nozzle sizes: 50µm, 70um, 100um | + | * available nozzle sizes: 70um, 100um |
| - | * up to 6 populations can be sorted at the same time (only available for 5mL, 2mL or 1.5mL tubes); | + | * up to 6 populations can be sorted at the same time (only available for 5 mL, 2 mL or 1.5 mL tubes) |
| * various collection vessels can be used: | * various collection vessels can be used: | ||
| - | * 5mL, 2mL, 1.5mL tubes (6-way sort possible); | + | * 5 mL, 2 mL, 1.5 mL tubes (6-way sort possible) |
| - | * 15mL tubes (2-way sort); | + | * 15 mL tubes (2-way sort) |
| - | * 50mL tubes (2-way sort); | + | * 50 mL tubes (2-way sort) |
| - | * slides: 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort) | + | * slides |
| - | * PCR tubes/strips/plates (1-way sort) | + | * 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort) |
| + | * PCR tubes/strips/plates (1-way sort) | ||
| * sort setup modes: | * sort setup modes: | ||
| * purity | * purity | ||
| * single cell (cloning) | * single cell (cloning) | ||
| - | * enrichment. | + | * enrichment |
| * **{{:aree:citometria:strumentazione:moflo-astrios-brochure.pdf |Brochure}}** | * **{{:aree:citometria:strumentazione:moflo-astrios-brochure.pdf |Brochure}}** | ||
| - | * **{{moflo-astrios-optical_conf.pdf |Optical configuration}}** | + | |
| + | \\ | ||
| + | |||
| + | ==== Optical Configuration ==== | ||
| + | |||
| + | ^ **Violet Laser** \\ <wrap lo>405 nm</wrap> ^ **Blue Laser** \\ <wrap lo>488 nm</wrap> ^ **Yellow Laser** \\ <wrap lo>561 nm</wrap> ^ **Red Laser** \\ <wrap lo>640 nm</wrap> ^ | ||
| + | | 448/59 \\ 546/20 \\ 620/29 \\ 671/30 | 488/6 \\ 513/26 \\ 664/22 \\ 710/45 | 579/19 \\ 614/20 \\ 692/75 \\ 795/70 | 671/30 \\ 722/44 \\ 795/70 | | ||
| \\ | \\ | ||
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| /* > FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)// */ | /* > FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)// */ | ||
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