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--- en:aree:citometria:strumentazione:cell_sorter [2019/06/27 15:41]
Gianluca Frustagli Added MoFlo Astrios Cell Sorter.
+++ en:aree:citometria:strumentazione:cell_sorter [2019/07/31 14:41] (current)
Gianluca Frustagli
@@ Line 1: / Line 1: @@
+~~NOTOC~~
 **Citometry Area**
 ====== Cell Sorter ======
-===== MoFlo Astrios =====
+===== MoFlo Astrios EQ =====
-Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scatter parameters (1 side scatter detectors and 2 forward scatter detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system.
+{{ :aree:citometria:strumentazione:moflo-astrios.jpeg?300|MoFlo Astrios}} Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scattering parameters (1 side scattering detectors and 2 forward scattering detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system.
 ==== Technical specifications ====
-  * Jet-in-air system;
+  * jet-in-air system
-  * Temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.);
+  * temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.)
-  * Available nozzle sizes: 50µm, 70um, 100um;
+  * available nozzle sizes: 70um, 100um
-  * Up to 6 populations can be sorted at the same time (only available for 5mL, 2mL or 1.5mL tubes);
+  * up to 6 populations can be sorted at the same time (only available for 5 mL, 2 mL or 1.5 mL tubes)
-  * Various collection vessels can be used:
+  * various collection vessels can be used:
-    * 5mL, 2mL, 1.5mL tubes (6-way sort possible);
+    * 5 mL, 2 mL, 1.5 mL tubes (6-way sort possible)
-    * 15mL tubes (2-way sort);
+    * 15 mL tubes (2-way sort)
-    * 50mL tubes (2-way sort);
+    * 50 mL tubes (2-way sort)
-    * slides;
+    * slides
-    * 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort);
+    * 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort)
-    * PCR tubes/strips/plates (1-way sort);
+    * PCR tubes/strips/plates (1-way sort)
-    * sort setup modes: purity, single cell (cloning), enrichment.
+  * sort setup modes:
+    * purity
+    * single cell (cloning)
+    * enrichment
+  * **{{:aree:citometria:strumentazione:moflo-astrios-brochure.pdf |Brochure}}**
+\\
+==== Optical Configuration ====
+^  **Violet Laser** \\ <wrap lo>405 nm</wrap>    ^  **Blue Laser** \\ <wrap lo>488 nm</wrap>  ^  **Yellow Laser** \\ <wrap lo>561 nm</wrap>  ^  **Red Laser** \\ <wrap lo>640 nm</wrap>    ^
+|  448/59 \\ 546/20 \\ 620/29 \\ 671/30          |  488/6 \\ 513/26 \\ 664/22 \\ 710/45       |  579/19 \\ 614/20 \\ 692/75 \\ 795/70        |  671/30 \\ 722/44 \\ 795/70                 |
+\\
 ===== FACSAria I - BD Biosciences =====
@@ Line 36: / Line 53: @@
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