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en:aree:citometria:strumentazione:cell_sorter [2018/08/08 15:04] Gianluca Frustagli |
en:aree:citometria:strumentazione:cell_sorter [2019/07/31 14:41] (current) Gianluca Frustagli |
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| + | ~~NOTOC~~ | ||
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| **Citometry Area** | **Citometry Area** | ||
| ====== Cell Sorter ====== | ====== Cell Sorter ====== | ||
| + | |||
| + | ===== MoFlo Astrios EQ ===== | ||
| + | |||
| + | {{ :aree:citometria:strumentazione:moflo-astrios.jpeg?300|MoFlo Astrios}} Our MoFlo Astrios EQ is a 4-laser cell sorter that can measure at the same time up to 15 fluorescence parameters and 3 light scattering parameters (1 side scattering detectors and 2 forward scattering detectors, all of them being photomultipliers, are available on the EQ update). The instrument has an aerosol containment system. | ||
| + | |||
| + | ==== Technical specifications ==== | ||
| + | |||
| + | * jet-in-air system | ||
| + | * temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.) | ||
| + | * available nozzle sizes: 70um, 100um | ||
| + | * up to 6 populations can be sorted at the same time (only available for 5 mL, 2 mL or 1.5 mL tubes) | ||
| + | * various collection vessels can be used: | ||
| + | * 5 mL, 2 mL, 1.5 mL tubes (6-way sort possible) | ||
| + | * 15 mL tubes (2-way sort) | ||
| + | * 50 mL tubes (2-way sort) | ||
| + | * slides | ||
| + | * 384w, 96w, 48w, 24w, 12w, 6w plates (1-way sort) | ||
| + | * PCR tubes/strips/plates (1-way sort) | ||
| + | * sort setup modes: | ||
| + | * purity | ||
| + | * single cell (cloning) | ||
| + | * enrichment | ||
| + | |||
| + | * **{{:aree:citometria:strumentazione:moflo-astrios-brochure.pdf |Brochure}}** | ||
| + | |||
| + | \\ | ||
| + | |||
| + | ==== Optical Configuration ==== | ||
| + | |||
| + | ^ **Violet Laser** \\ <wrap lo>405 nm</wrap> ^ **Blue Laser** \\ <wrap lo>488 nm</wrap> ^ **Yellow Laser** \\ <wrap lo>561 nm</wrap> ^ **Red Laser** \\ <wrap lo>640 nm</wrap> ^ | ||
| + | | 448/59 \\ 546/20 \\ 620/29 \\ 671/30 | 488/6 \\ 513/26 \\ 664/22 \\ 710/45 | 579/19 \\ 614/20 \\ 692/75 \\ 795/70 | 671/30 \\ 722/44 \\ 795/70 | | ||
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| + | |||
| + | \\ | ||
| ===== FACSAria I - BD Biosciences ===== | ===== FACSAria I - BD Biosciences ===== | ||
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| * Deposition on multiwell plates and slide | * Deposition on multiwell plates and slide | ||
| - | > FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)// | + | \\ \\ \\ \\ |
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| + | /* > FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)// */ | ||
