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en:aree:citometria:start [2018/08/06 16:24] Gianluca Frustagli English translation. |
en:aree:citometria:start [2025/02/14 20:25] (current) Gianluca Frustagli Update of toolset and contact person. |
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| - | > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// | ||
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| **Area** | **Area** | ||
| ====== Cytometry ====== | ====== Cytometry ====== | ||
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| - | The //Core Facility// performs cytometric flow analysis, cytofluorimetric or mass, at the single cell level. | + | The Cytometry Area is equipped with 4 conventional analyzers that allow analysis of up to 19 fluorescence parameters and, simultaneously, two scattering parameters; a spectral analyzer equipped with 5 lasers for the analysis of three scattering parameters and up to 64 fluorescence parameters; a //cell sorter// equipped with 4 lasers, capable of separating up to 6 cell populations based on a maximum of 15 fluorescence parameters. A dedicated area for cell cultures and sample preparation in sterile conditions for flow cytometric analyses. \\ |
| - | The Area is equipped with three analyzers that allow analysis of up to 10 fluorescence parameters, two cell sorter capable of separating up to four cell populations on the basis of 10 fluorescence parameters and a mass cytometer (CyTOF) that allows to perform multiparametric analysis between 25-35 parameters (up to a theoretical maximum of 90), based on the use of antibodies conjugated to rare metals. | + | |
| Cytofluorimetric analysis can be conducted in High-Throughput mode. | Cytofluorimetric analysis can be conducted in High-Throughput mode. | ||
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| The cytofluorimetry and mass cytometry //Core Facility// aims to promote access to and use of these technologies for ISS researchers and to actively promote both the collaboration and the provision of services to users outside the Institute. | The cytofluorimetry and mass cytometry //Core Facility// aims to promote access to and use of these technologies for ISS researchers and to actively promote both the collaboration and the provision of services to users outside the Institute. | ||
| - | > Contact: [[massimo.sanchez@iss.it|Massimo Sanchez]] | + | \\ |
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| + | > Contact: [[mario.picozza@iss.it|Mario Picozza]] | ||
| > ☎ **+39 06 4990 2576-2550** | > ☎ **+39 06 4990 2576-2550** | ||
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| + | /* > FIXME **To be revised.**\\ //(remove this paragraph once finished)// */ | ||
