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en:aree:citometria:servizi:start [2019/05/16 01:41]
Gianluca Frustagli Realignment with italian version.
en:aree:citometria:servizi:start [2026/01/07 10:29] (current)
Luca Pasquini
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 ====== Main services offered ====== ====== Main services offered ======
  
-  * **Cytometric analysis ​service on single cell** ​(multiparametric), cell separation and data analysis.+  * **Cytometric analysis ​at single cell level**, cell separation and data analysis.
  
   * **Multiparametric analysis:**   * **Multiparametric analysis:**
-    * in cytofluorimetry, ​up to 10 parameters;​ +    * up to 19 parameters ​in conventional cytometry mode
-    * in cytofluorimetry ​in High-Throughput mode; +    * Cytometric analysis ​in High-Throughput mode; 
-    * in mass cytometry, upto 35 parameters.+    * up to 45 parameters ​in spectral ​cytometry ​mode.
  
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 {{:​aree:​citometria:​servizi:​esempio_immunofenotipo-4.png?​150|Immunophenotype of mononuclear cells of human peripheral blood - 4}}{{:​aree:​citometria:​servizi:​esempio_immunofenotipo-5.png?​150|Immunophenotype of mononuclear cells of human peripheral blood - 5}}{{:​aree:​citometria:​servizi:​esempio_immunofenotipo-6.png?​150|Immunophenotype of mononuclear cells of human peripheral blood - 6}} {{:​aree:​citometria:​servizi:​esempio_immunofenotipo-4.png?​150|Immunophenotype of mononuclear cells of human peripheral blood - 4}}{{:​aree:​citometria:​servizi:​esempio_immunofenotipo-5.png?​150|Immunophenotype of mononuclear cells of human peripheral blood - 5}}{{:​aree:​citometria:​servizi:​esempio_immunofenotipo-6.png?​150|Immunophenotype of mononuclear cells of human peripheral blood - 6}}
  
-<wrap lo>​**Immunophenotype of mononuclear cells of human peripheral blood**</​wrap>​+<wrap lo>​**Immunophenotype of mononuclear cells of human peripheral blood in conventional cytometry**</​wrap>​
  
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-  * **Cell separation:​** ​it is possible to obtain ​highly purified cell populations, coming ​from various types of human and animal tissues ​by cell sorting.+  * **Cell separation:​** ​obtaining ​highly purified cell populations from various types of human and animal tissues.
 </​WRAP>​ </​WRAP>​
 <WRAP half column> <WRAP half column>
 {{:​aree:​citometria:​servizi:​cell_sorting-1.png?​0x120|Cell sorting}}{{:​aree:​citometria:​servizi:​cell_sorting-2.jpeg?​0x120|Cell sorting}}{{:​aree:​citometria:​servizi:​cell_sorting-3.png?​0x120|Cell sorting}} {{:​aree:​citometria:​servizi:​cell_sorting-1.png?​0x120|Cell sorting}}{{:​aree:​citometria:​servizi:​cell_sorting-2.jpeg?​0x120|Cell sorting}}{{:​aree:​citometria:​servizi:​cell_sorting-3.png?​0x120|Cell sorting}}
  
-<wrap lo>​**CELL SORTING:​** ​On the left of the figure: diagram of the separation principle. -- In the center and on the right: the internal display of the FACS Aria I (detail on the deflection plates and the 4 channels of the sorting) and the window of the DIVA7 software for the management of the sorting.</​wrap>​+<wrap lo>​**CELL SORTING:​** ​xxxxxxx.</​wrap>​
 </​WRAP>​ </​WRAP>​
 </​WRAP>​ </​WRAP>​
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-  * **Data analysis:** Diva, Kaluza, FlowJo software+  * **Data analysis:** Diva, CytexpertKaluza and FlowJo software
  
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-{{:​aree:​citometria:​servizi:​esempio_cell_cycle-1.png?​150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 1}}{{:​aree:​citometria:​servizi:​esempio_cell_cycle-2.png?​150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 2}}{{:​aree:​citometria:​servizi:​esempio_cell_cycle-3.png?​150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 3}}+{{:​aree:​citometria:​servizi:​esempio_cell_cycle-1.png?​150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo ​- 1}}{{:​aree:​citometria:​servizi:​esempio_cell_cycle-2.png?​150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo ​- 2}}{{:​aree:​citometria:​servizi:​esempio_cell_cycle-3.png?​150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo ​- 3}}
  
 {{:​aree:​citometria:​servizi:​esempio_cell_cycle-4.jpeg?​450|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 4}} {{:​aree:​citometria:​servizi:​esempio_cell_cycle-4.jpeg?​450|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 4}}
  
-<wrap lo>​Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo</​wrap>​+<wrap lo>​Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo</​wrap>​
  
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-  * **Design and development of cytometric panels** ​nd preparation of experimental protocols in agreement with the researchers involved((**NOTE:​** ​Cellular ​separations are performed only by laboratory personnel.)).+  * **Design and development of cytometric panels** ​and preparation of experimental protocols in agreement with the researchers involved((**NOTE:​** ​Cell separations are performed only by facility staff.)).
  
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 /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */