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--- en:aree:citometria:servizi:start [2018/08/06 19:17]
Gianluca Frustagli
+++ en:aree:citometria:servizi:start [2026/01/07 10:29] (current)
Luca Pasquini
@@ Line 1: / Line 1: @@
-> FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)//
 **Citometry Area**
 ====== Main services offered ======
-  * **Cytometric analysis service on single cell** (multiparametric), cell separation and data analysis.
+  * **Cytometric analysis at single cell level**, cell separation and data analysis.
   * **Multiparametric analysis:**
-    * in cytofluorimetry, up to 10 parameters;
+    * up to 19 parameters in conventional cytometry mode;
-    * in cytofluorimetry in High-Throughput mode;
+    * Cytometric analysis in High-Throughput mode;
-    * in mass cytometry, currently between 25 and 30 parameters.
+    * up to 45 parameters in spectral cytometry mode.
 \\
@@ Line 16: / Line 14: @@
 {{:aree:citometria:servizi:esempio_immunofenotipo-4.png?150|Immunophenotype of mononuclear cells of human peripheral blood - 4}}{{:aree:citometria:servizi:esempio_immunofenotipo-5.png?150|Immunophenotype of mononuclear cells of human peripheral blood - 5}}{{:aree:citometria:servizi:esempio_immunofenotipo-6.png?150|Immunophenotype of mononuclear cells of human peripheral blood - 6}}
-<wrap lo>**Immunophenotype of mononuclear cells of human peripheral blood**</wrap>
+<wrap lo>**Immunophenotype of mononuclear cells of human peripheral blood in conventional cytometry**</wrap>
 \\
@@ Line 27: / Line 25: @@
 \\ \\ \\
-  * **Cell separation:** it is possible to obtain highly purified cell populations, coming from various types of human and animal tissues by cell sorting.
+  * **Cell separation:** obtaining highly purified cell populations from various types of human and animal tissues.
 </WRAP>
 <WRAP half column>
 {{:aree:citometria:servizi:cell_sorting-1.png?0x120|Cell sorting}}{{:aree:citometria:servizi:cell_sorting-2.jpeg?0x120|Cell sorting}}{{:aree:citometria:servizi:cell_sorting-3.png?0x120|Cell sorting}}
-<wrap lo>**CELL SORTING:** On the left of the figure: diagram of the separation principle. -- In the center and on the right: the internal display of the FACS Aria I (detail on the deflection plates and the 4 channels of the sorting) and the window of the DIVA7 software for the management of the sorting.</wrap>
+<wrap lo>**CELL SORTING:** xxxxxxx.</wrap>
 </WRAP>
 </WRAP>
@@ Line 40: / Line 38: @@
 \\
-  * **Data analysis:** Diva, Kaluza, FlowJo software
+  * **Data analysis:** Diva, Cytexpert, Kaluza and FlowJo software
 \\
-{{:aree:citometria:servizi:esempio_cell_cycle-1.png?150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 1}}{{:aree:citometria:servizi:esempio_cell_cycle-2.png?150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 2}}{{:aree:citometria:servizi:esempio_cell_cycle-3.png?150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 3}}
+{{:aree:citometria:servizi:esempio_cell_cycle-1.png?150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo - 1}}{{:aree:citometria:servizi:esempio_cell_cycle-2.png?150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo - 2}}{{:aree:citometria:servizi:esempio_cell_cycle-3.png?150|Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo - 3}}
 {{:aree:citometria:servizi:esempio_cell_cycle-4.jpeg?450|Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo - 4}}
-<wrap lo>Example of analysis of the PI/BrDU cell cycle with Kaluza and FloJo</wrap>
+<wrap lo>Example of analysis of the PI/BrDU cell cycle with Kaluza and FlowJo</wrap>
 \\
@@ Line 54: / Line 52: @@
 \\
-  * **Design and development of cytometric panels** nd preparation of experimental protocols in agreement with the researchers involved((**NOTE:** Cellular separations are performed only by laboratory personnel.)).
+  * **Design and development of cytometric panels** and preparation of experimental protocols in agreement with the researchers involved((**NOTE:** Cell separations are performed only by facility staff.)).
 \\
+/* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */