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2026-05-01T03:51:35+02:00Grandi Strumentazioni e Core Facilities (FAST)
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EPR Area
Surface Plasmon Resonance (SPR - Biacore)
Service requestAbstractMethodApplications
Applications
Typical applications are:
affinity and kinetics;quantitative titration of analyte;specificity;compliance to a Reference Standard;termodynamics.
Example: kinetic test
Here is presented an example of consecutive injections analysis. As presented above the instrument can perform sequential analysis of the kinetic. Here we stratified a specific epitope to the sensor chip and then we tested …text/html2024-04-19T19:52:58+02:00Surface Plasmon Resonance (SPR - Biacore)
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EPR Area
Surface Plasmon Resonance (SPR - Biacore)
Service requestAbstractMethodApplications
Methodology
The SPR is substantially a very precise molecular scale, able to report the binding of a single amino acid (100 Da), with a sensibility of few pg/mm² in a pM range of the bulk.
If polarized light is focalized on a thin gold layer starting from a condition of total reflection and then the angle is let to be changed, measuring the intensity of the reflected beam it appends that at a specif…text/html2025-05-23T02:33:48+02:00Surface Plasmon Resonance (SPR - Biacore)
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EPR Area
Surface Plasmon Resonance (SPR - Biacore)
Service requestAbstractMethodApplications
After clicking on “Send request” it's necessary to wait few seconds for the operation to complete.Information on data processing.text/html2024-04-24T19:29:41+02:00Surface Plasmon Resonance (SPR - Biacore)
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EPR Area
Surface Plasmon Resonance (SPR - Biacore)
Service requestAbstractMethodApplications
Abstract
The Surface Plasmon Resonance (SPR) technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²).