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en:aree:proteomica:rppa:start [2018/03/01 19:55] Gianluca Frustagli Added translation (to be revised) |
en:aree:proteomica:rppa:start [2019/06/18 16:48] (current) Gianluca Frustagli |
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| - | > FIXME **To be revised**\\ //(remove this paragraph once the translation is finished)// | ||
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| **Proteomics Area** | **Proteomics Area** | ||
| + | |||
| ====== Reverse-Phase Protein microArrays (RPPA) ====== | ====== Reverse-Phase Protein microArrays (RPPA) ====== | ||
| <WRAP tabs> | <WRAP tabs> | ||
| - | * [[:aree:proteomica:rppa:richiesta|Service request]] | + | * [[en:aree:proteomica:rppa:richiesta|Service request]] |
| - | * [[:aree:proteomica:rppa:protocolli|Protocols]] | + | * [[en:aree:proteomica:rppa:protocolli|Protocols]] |
| - | * [[:aree:proteomica:rppa:bibliografia|Bibliography]] | + | * [[en:aree:proteomica:rppa:bibliografia|Bibliography]] |
| - | * [[:aree:proteomica:rppa:link_tutorial|Links & Tutorial]] | + | * [[en:aree:proteomica:rppa:link_tutorial|Links & Tutorials]] |
| - | * [[:aree:proteomica:rppa:faq|FAQ]] | + | * [[en:aree:proteomica:rppa:faq|FAQ]] |
| </WRAP> | </WRAP> | ||
| Line 23: | Line 22: | ||
| * a laser-capture microdissector with infrared laser, to cut user-selected portions of tissue from frozen tissue sections and minimize photo-damage of (phospho-)proteins; | * a laser-capture microdissector with infrared laser, to cut user-selected portions of tissue from frozen tissue sections and minimize photo-damage of (phospho-)proteins; | ||
| * a robotic, contact pin arrayer that allows micro-deposition of hundreds of samples on nitrocellulose-coated slides; | * a robotic, contact pin arrayer that allows micro-deposition of hundreds of samples on nitrocellulose-coated slides; | ||
| - | * an Autostainer, to allow automated immunostaining and signal amplification; | + | * an //autostainer//, to allow automated immunostaining and signal amplification; |
| * a laser scanner to digitalize the fluorescent signal from spotted samples on the slide surface; | * a laser scanner to digitalize the fluorescent signal from spotted samples on the slide surface; | ||
| * a dedicated image analysis software for automated detection, quantification and normalization of signal. | * a dedicated image analysis software for automated detection, quantification and normalization of signal. | ||
| - | <wrap hi>Such a pipeline of instruments makes the RPPA unit at ISS an unparalleled laboratory implementation in Italy</wrap>. Moreover, the RPPA facility hosts a bank of __{{:aree:proteomica:rppa:validated_antibodies-rppa-iss.xlsx|> 400 antibodies}}__ directed against proteins, phosho-proteins and other post-translational modifications (__{{:aree:proteomica:rppa:pathway_anticorpi-rppa-iss.xlsx|Table 1}}__) The number of antibodies undergoing validation for RPPA use is increasing, based on the requirement of novel project collaborations. | + | <wrap hi>Such a pipeline of instruments makes the RPPA unit at ISS an unparalleled laboratory implementation in Italy</wrap>. |
| - | The RPPA unit at ISS has diverse internal collaborations as well as other national colaborators such as the National Cancer Institute in Milan and in Rome, the University of Rome "Tor Vergata", the "Catholic University of Rome" and the University of Palermo. | + | Moreover, the RPPA facility hosts a bank of __{{aree:proteomica:rppa:validated_antibodies-rppa-iss.xlsx|> 400 antibodies}}__ directed against proteins, phosho-proteins and other post-translational modifications (__{{aree:proteomica:rppa:pathway_anticorpi-rppa-iss.xlsx|Table 1}}__) The number of antibodies undergoing validation for RPPA use is increasing, based on the requirement of novel project collaborations. |
| + | |||
| + | The RPPA unit at ISS has diverse internal collaborations as well as other national collaborators such as the National Cancer Institute in Milan and in Rome, the University of Rome "Tor Vergata", the "Catholic University of Rome" and the University of Palermo. | ||
| The RPPA unit at ISS may provide technical and scientific support in experimental design, samples preparation and data analysis. | The RPPA unit at ISS may provide technical and scientific support in experimental design, samples preparation and data analysis. | ||
| - | In order to access the service, please complete the online [[aree:proteomica:rppa:richiesta|Request Form]]. Our personnel will contact you to arrange an informative discussion and brainstorming. | + | \\ |
| + | |||
| + | === To ask for the service: === | ||
| + | |||
| + | - preliminary contact the [[chi_siamo|RPPA unit staff]]; | ||
| + | - fill out the online form '**[[richiesta|Service Request]]**' briefly illustrating the scientific problem, type and number of samples etc.; | ||
| + | - plan a meeting with the unit staff to discuss methods of analysis, costs, timing etc. | ||
| + | |||
| + | \\ | ||
| > Contact: [[michele.signore@iss.it|Michele Signore]] | > Contact: [[michele.signore@iss.it|Michele Signore]] | ||
| > ☎ **+39 06 4990 4453** | > ☎ **+39 06 4990 4453** | ||
| > Where we are: **GB-BS, room 7** | > Where we are: **GB-BS, room 7** | ||
| + | |||
| + | /* > FIXME **To be revised**\\ //(remove this paragraph once finished)// */ | ||
