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en:aree:proteomica:ms:linee_guida [2018/08/10 13:48] Gianluca Frustagli English translation. |
en:aree:proteomica:ms:linee_guida [2019/06/18 16:35] (current) Gianluca Frustagli [Polymers] |
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| **Proteomics Area** | **Proteomics Area** | ||
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| ====== MassSpec ====== | ====== MassSpec ====== | ||
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| * [[en:aree:proteomica:ms:protocolli|Protocols]] | * [[en:aree:proteomica:ms:protocolli|Protocols]] | ||
| * [[en:aree:proteomica:ms:bibliografia|Bibliography]] | * [[en:aree:proteomica:ms:bibliografia|Bibliography]] | ||
| - | * [[en:aree:proteomica:ms:link_tutorial|Link & Tutorial]] | + | * [[en:aree:proteomica:ms:link_tutorial|Links & Tutorials]] |
| * [[en:aree:proteomica:ms:faq|FAQ]] | * [[en:aree:proteomica:ms:faq|FAQ]] | ||
| </WRAP> | </WRAP> | ||
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| Polymers are the most harmful contaminants, since in addition to suppressing the signal they tend to stick to HPLC columns or capillaries, damaging the system or at least causing a serious memory effect on subsequent LC-MS strokes. Most polymers ionize peptides more easily and this means that even small amounts can be detrimental to the experiment. The polymers most frequently observed are various forms of PEG, present in some plastics; but also in soaps, hand creams (__wearing gloves is essential!__) and detergents. It is not always easy to trace the source of a contamination from polymers, but a certain source is the __Parafilm__, to avoid absolutely to close tubes or bottles of solvents, or gel trays etc. Furthermore, detergents should not be present in the samples to be subjected to MS, for this reason the analysis is always preceded by PAGE or chromatography. | Polymers are the most harmful contaminants, since in addition to suppressing the signal they tend to stick to HPLC columns or capillaries, damaging the system or at least causing a serious memory effect on subsequent LC-MS strokes. Most polymers ionize peptides more easily and this means that even small amounts can be detrimental to the experiment. The polymers most frequently observed are various forms of PEG, present in some plastics; but also in soaps, hand creams (__wearing gloves is essential!__) and detergents. It is not always easy to trace the source of a contamination from polymers, but a certain source is the __Parafilm__, to avoid absolutely to close tubes or bottles of solvents, or gel trays etc. Furthermore, detergents should not be present in the samples to be subjected to MS, for this reason the analysis is always preceded by PAGE or chromatography. | ||
| - | > FIXME **To be revised.**\\ //(remove this paragraph once finished)// | + | /* > FIXME **To be revised.**\\ //(remove this paragraph once finished)// */ |
