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en:aree:proteomica:biacore:start [2022/02/24 16:00] Gianluca Frustagli |
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| - | ~~NOTOC~~ | ||
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| - | **Proteomics Area** | ||
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| - | ====== Surface Plasmon Resonance (SPR - Biacore) ====== | ||
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| - | <WRAP tabs> | ||
| - | * [[en:aree:proteomica:biacore:start|Abstract]] | ||
| - | * [[en:aree:proteomica:biacore:metodica|Methodology]] | ||
| - | * [[en:aree:proteomica:biacore:applicazioni|Applications]] | ||
| - | </WRAP> | ||
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| - | ==== Abstract ==== | ||
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| - | The <wrap em>Surface Plasmon Resonance (SPR)</wrap> technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²). | ||
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| - | Utilizzando specifici chip, è possibile misurare interazioni di vario genere a seconda della macromolecola immobilizzata sul supporto, indagando così interazioni proteina-proteina, proteina-lipide, proteina-acido nucleico e lipide-lipide. La tecnica ha applicazioni in diversi ambiti, quali Biochimica, Biologia molecolare, Virologia, Tossicologia, Lipidomica, e controllo di qualità di farmaci biotecnologici. | ||
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| - | According to the specific macromolecule stratified on the chip, it is possible to test mixed interactions such as protein to protein, protein to lipid, lipid to lipid and nucleic acid to protein. Applications span several fields like biochemistry, molecular biology, virology, toxicology, lipidomic as well in GMP. | ||
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| - | <WRAP group> /* 1st GROUP OF COLUMNS - BEGIN */ | ||
| - | <WRAP half column> /* FIRST COLUMN - BEGIN */ | ||
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| - | <WRAP centeralign>**Proteins immobilization**</WRAP> | ||
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| - | {{ :aree:proteomica:biacore:proteins_immobilization.png?300 |}} | ||
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| - | </WRAP> /* FIRST COLUMN - END */ | ||
| - | <WRAP half column> /* SECOND COLUMN - BEGIN */ | ||
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| - | \\ | ||
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| - | <WRAP centeralign>**Tagged recombinant proteins immobilization**</WRAP> | ||
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| - | {{ :aree:proteomica:biacore:tagged_recombinant_proteins_immobilization.png?120 |}} | ||
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| - | </WRAP> /* SECOND COLUMN - END */ | ||
| - | </WRAP> /* 1st GROUP OF COLUMNS - END */ | ||
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| - | <WRAP group> /* 2nd GROUP OF COLUMNS - BEGIN */ | ||
| - | <WRAP half column> /* FIRST COLUMN - BEGIN */ | ||
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| - | \\ | ||
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| - | <WRAP centeralign>**Nucleic acid immobilization**</WRAP> | ||
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| - | {{ :aree:proteomica:biacore:nucleic_acid_immobilization.png?300 |}} | ||
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| - | </WRAP> /* FIRST COLUMN - END */ | ||
| - | <WRAP half column> /* SECOND COLUMN - BEGIN */ | ||
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| - | \\ | ||
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| - | <WRAP centeralign>**Lipids immobilization**</WRAP> | ||
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| - | {{ :aree:proteomica:biacore:lipids_immobilization1.png?300 |}} | ||
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| - | {{ :aree:proteomica:biacore:lipids_immobilization2.png?300 |}} | ||
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| - | {{ :aree:proteomica:biacore:lipids_immobilization3.png?300 |}} | ||
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| - | </WRAP> /* SECOND COLUMN - END */ | ||
| - | </WRAP> /* 2nd GROUP OF COLUMNS - END */ | ||
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| - | \\ | ||
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| - | <wrap em>We make use of the **Biacore X100** (Cytiva) instrument:</wrap> {{ :aree:proteomica:biacore:strumento.jpeg?260}} | ||
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| - | [[https://www.cytivalifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/systems/biacore-x100-p-05730]] | ||
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| - | \\ \\ \\ \\ \\ | ||
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| - | > Contact: [[marcello.belfiore@iss.it|Marcello Belfiore]] | ||
| - | > ☎ **+39 06 4990 3046** | ||
| - | > Where I am: building **10**, floor **D**, room **9** | ||
