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en:aree:proteomica:biacore:start [2022/02/24 15:50] Gianluca Frustagli created |
en:aree:proteomica:biacore:start [2022/11/18 20:47] Gianluca Frustagli |
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| - | > FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)// | ||
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| ~~NOTOC~~ | ~~NOTOC~~ | ||
| - | **Proteomic Area** | + | **Proteomics Area** |
| ====== Surface Plasmon Resonance (SPR - Biacore) ====== | ====== Surface Plasmon Resonance (SPR - Biacore) ====== | ||
| <WRAP tabs> | <WRAP tabs> | ||
| - | * [[:aree:proteomica:biacore:start|Abstract]] | + | * [[en:aree:proteomica:biacore:richiesta|Service request]] |
| - | * [[:aree:proteomica:biacore:metodica|Methodology]] | + | * [[en:aree:proteomica:biacore:start|Abstract]] |
| - | * [[:aree:proteomica:biacore:applicazioni|Applications]] | + | * [[en:aree:proteomica:biacore:metodica|Method]] |
| + | * [[en:aree:proteomica:biacore:applicazioni|Applications]] | ||
| </WRAP> | </WRAP> | ||
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| The <wrap em>Surface Plasmon Resonance (SPR)</wrap> technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²). | The <wrap em>Surface Plasmon Resonance (SPR)</wrap> technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²). | ||
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| - | Utilizzando specifici chip, è possibile misurare interazioni di vario genere a seconda della macromolecola immobilizzata sul supporto, indagando così interazioni proteina-proteina, proteina-lipide, proteina-acido nucleico e lipide-lipide. La tecnica ha applicazioni in diversi ambiti, quali Biochimica, Biologia molecolare, Virologia, Tossicologia, Lipidomica, e controllo di qualità di farmaci biotecnologici. | ||
| According to the specific macromolecule stratified on the chip, it is possible to test mixed interactions such as protein to protein, protein to lipid, lipid to lipid and nucleic acid to protein. Applications span several fields like biochemistry, molecular biology, virology, toxicology, lipidomic as well in GMP. | According to the specific macromolecule stratified on the chip, it is possible to test mixed interactions such as protein to protein, protein to lipid, lipid to lipid and nucleic acid to protein. Applications span several fields like biochemistry, molecular biology, virology, toxicology, lipidomic as well in GMP. | ||
| - | <WRAP group> /* INIZIO 1° GRUPPO DI COLONNE */ | + | <WRAP group> /* 1st GROUP OF COLUMNS - BEGIN */ |
| - | <WRAP half column> /* INIZIO PRIMA COLONNA */ | + | <WRAP half column> /* FIRST COLUMN - BEGIN */ |
| \\ | \\ | ||
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| {{ :aree:proteomica:biacore:proteins_immobilization.png?300 |}} | {{ :aree:proteomica:biacore:proteins_immobilization.png?300 |}} | ||
| - | </WRAP> /* FINE PRIMA COLONNA */ | + | </WRAP> /* FIRST COLUMN - END */ |
| - | <WRAP half column> /* INIZIO SECONDA COLONNA */ | + | <WRAP half column> /* SECOND COLUMN - BEGIN */ |
| \\ | \\ | ||
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| {{ :aree:proteomica:biacore:tagged_recombinant_proteins_immobilization.png?120 |}} | {{ :aree:proteomica:biacore:tagged_recombinant_proteins_immobilization.png?120 |}} | ||
| - | </WRAP> /* FINE SECONDA COLONNA */ | + | </WRAP> /* SECOND COLUMN - END */ |
| - | </WRAP> /* FINE 1° GRUPPO DI COLONNE */ | + | </WRAP> /* 1st GROUP OF COLUMNS - END */ |
| - | <WRAP group> /* INIZIO 2° GRUPPO DI COLONNE */ | + | <WRAP group> /* 2nd GROUP OF COLUMNS - BEGIN */ |
| - | <WRAP half column> /* INIZIO PRIMA COLONNA */ | + | <WRAP half column> /* FIRST COLUMN - BEGIN */ |
| \\ | \\ | ||
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| {{ :aree:proteomica:biacore:nucleic_acid_immobilization.png?300 |}} | {{ :aree:proteomica:biacore:nucleic_acid_immobilization.png?300 |}} | ||
| - | </WRAP> /* FINE PRIMA COLONNA */ | + | </WRAP> /* FIRST COLUMN - END */ |
| - | <WRAP half column> /* INIZIO SECONDA COLONNA */ | + | <WRAP half column> /* SECOND COLUMN - BEGIN */ |
| \\ | \\ | ||
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| {{ :aree:proteomica:biacore:lipids_immobilization3.png?300 |}} | {{ :aree:proteomica:biacore:lipids_immobilization3.png?300 |}} | ||
| - | </WRAP> /* FINE SECONDA COLONNA */ | + | </WRAP> /* SECOND COLUMN - END */ |
| - | </WRAP> /* FINE 2° GRUPPO DI COLONNE */ | + | </WRAP> /* 2nd GROUP OF COLUMNS - END */ |
| \\ | \\ | ||
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| \\ \\ \\ \\ \\ | \\ \\ \\ \\ \\ | ||
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| + | === To ask for the service: === | ||
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| + | - preliminary contact the Biacore unit staff; | ||
| + | - fill out the online form '**[[richiesta|Service Request]]**' briefly illustrating the scientific problem. | ||
| + | |||
| + | \\ | ||
| > Contact: [[marcello.belfiore@iss.it|Marcello Belfiore]] | > Contact: [[marcello.belfiore@iss.it|Marcello Belfiore]] | ||
| > ☎ **+39 06 4990 3046** | > ☎ **+39 06 4990 3046** | ||
| > Where I am: building **10**, floor **D**, room **9** | > Where I am: building **10**, floor **D**, room **9** | ||
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