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en:aree:microscopia:confocale:start [2018/09/05 14:36] Gianluca Frustagli Added advice for service request process. |
en:aree:microscopia:confocale:start [2022/03/25 19:09] |
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| - | **Microscopy Area** | ||
| - | ====== Confocal Microscopy ====== | ||
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| - | {{ :aree:microscopia:confocale:banner.png?700 |}} | ||
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| - | Confocal Laser Scanning Microscopy (CLSM) is a standard technology with the following advantages over conventional widefield microscopy: | ||
| - | * Removal of light from out-of-focus planes; | ||
| - | * Improved lateral (XY) and axial (Z) resolution; | ||
| - | * Ability to perform a series of optical sections from specimens; | ||
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| - | The confocal microscope is a valuable tool for research because the system is able to obtain images with clarity and reasonable resolution, thus enabling 3-D reconstruction of the specimen from optical sections or series of images obtained. | ||
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| - | Currently, the Unit has 1 **Leica TCS SP2** upright microscope equipped with a laser diode at 405nm, Argon/Kripton 488nm, Helium/Neon 543-594 and 633 nm, with 10x, 20x, 40x, 63x and 100x objectives. | ||
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| - | The Unit also provides equipment for sample preparation and maintenance prior to imaging, as well as resources for the data processing. | ||
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| - | **The 3D-imaging properties of the CLSM make it an ideal tool to study cell biology and life sciences.** | ||
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| - | /* NOTA: l'URL specificato all'interno del codice HTML non è un riferimento assoluto ma risulta effettivamente relativo alla directory di installazione di DokuWiki sul server. */ | ||
| - | <html> | ||
| - | <table style="background-image: url('lib/exe/fetch.php?media=aree:microscopia:confocale:composizione.png'); background-repeat: no-repeat; background-size: 100%;"> | ||
| - | <tr><td colspan="3" style="height: 230px;"> </td></tr> | ||
| - | <tr><td style="width: 25%;"> </td><td> | ||
| - | <p>Key applications areas include:<p> | ||
| - | <ul> | ||
| - | <li>localization and co-localization in cells and tissues;</li> | ||
| - | <li>cytoskeleton and organelle functions;</li> | ||
| - | <li>vesicles trafficking;</li> | ||
| - | <li>membrane and ion probes;</li> | ||
| - | <li>cell migration and invasion;</li> | ||
| - | <li>bioluminescent proteins;</li> | ||
| - | <li>epitope tagging;</li> | ||
| - | <li>cellular metabolism, oxidative reactions;</li> | ||
| - | <li>Cytotoxicity;</li> | ||
| - | <li>Stem cell research;</li> | ||
| - | </ul> | ||
| - | </td><td style="width: 25%;"> </td></tr> | ||
| - | </table> | ||
| - | </html> | ||
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| - | \\ | ||
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| - | In addition, the Unit also provides basic protocols and all reagents needed to perform manually routine histological stainings (e.g. H&E and immune staining). | ||
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| - | Personnel of the Unit assist scientist throughout the full process of microscopy experiments: design projects, processing data and analyzing images, and interpreting and shaping final results. | ||
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| - | === To ask for the service: === | ||
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| - | - preliminary contact the Confocal Microscopy unit staff; | ||
| - | - fill out the online form '**[[richiesta|Service Request]]**' briefly illustrating the scientific problem, type and number of samples, etc. ; | ||
| - | - plan a meeting with the unit staff to discuss methods, costs, timing etc. | ||
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| - | > Contacts: | ||
| - | > [[serena.cecchetti@iss.it|Serena Cecchetti]], [[francesca.spadaro@iss.it|Francesca Spadaro]], ☎ **+39 06 4990 2966** | ||
| - | > [[paola.sestili@iss.it|Paola Sestili]], ☎ **+39 06 4990 3696** | ||
| - | > Where we are: **building 20, floor 2, room 6** | ||
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| - | > FIXME **To be revised.**\\ //(remove this paragraph once finished)// | ||
