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en:aree:in_collaborazione:tossine_algali:metodi [2023/02/15 19:42] Gianluca Frustagli [Service for the identification and quantification of algal toxins in aquatic and tissue matrices] |
en:aree:in_collaborazione:tossine_algali:metodi [2023/03/07 14:38] (current) Gianluca Frustagli |
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| - | <WRAP todo> | ||
| - | ==== Under construction ==== | ||
| - | </WRAP> | ||
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| **Services and tools in collaboration** | **Services and tools in collaboration** | ||
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| The currently available test (ABRAXIS, USA) is a direct competitive ELISA based on the recognition of BMAA by specific antibodies. BMAA, if present in a sample, and a BMAA-HRP analogue compete for the binding sites of rabbit anti-BMAA antibodies in solution. The BMAA antibodies are then bound by a second immobilized antibody on the wells of the microtitre plate. After a wash step and addition of substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of BMAA present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA microplate reader. Sample concentrations are determined by interpolation using the standard curve constructed with each test. The LOD for this test, based on MC-LR, is 4 μg/L. The coefficients of variation (CV%) for the standards are less than 10%; for samples below 15%. | The currently available test (ABRAXIS, USA) is a direct competitive ELISA based on the recognition of BMAA by specific antibodies. BMAA, if present in a sample, and a BMAA-HRP analogue compete for the binding sites of rabbit anti-BMAA antibodies in solution. The BMAA antibodies are then bound by a second immobilized antibody on the wells of the microtitre plate. After a wash step and addition of substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of BMAA present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA microplate reader. Sample concentrations are determined by interpolation using the standard curve constructed with each test. The LOD for this test, based on MC-LR, is 4 μg/L. The coefficients of variation (CV%) for the standards are less than 10%; for samples below 15%. | ||
| + | /* > FIXME **To be revised.**\\ //(remove this paragraph once finished)// */ | ||
