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en:aree:epr:altre_risorse:start [2020/10/30 18:40] Paola Fattibene |
en:aree:epr:altre_risorse:start [2024/04/24 19:24] (current) Gianluca Frustagli [Surface Plasmon Resonance (SPR - Biacore)] New contact. |
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====== Other instrumental resources ====== | ====== Other instrumental resources ====== | ||
- | **Immuno-spin trapping** | + | =====Immuno-spin trapping===== |
The increase of **"Oxidant Reactive Species" (ROS)** produced under conditions of oxidative stress can induce reversible/irreversible damage in the target molecules through the formation of radical intermediates centered on the target molecules. However, many of these radicals have a short half-life and their monitoring becomes problematic with both direct EPR and spin trapping technique. | The increase of **"Oxidant Reactive Species" (ROS)** produced under conditions of oxidative stress can induce reversible/irreversible damage in the target molecules through the formation of radical intermediates centered on the target molecules. However, many of these radicals have a short half-life and their monitoring becomes problematic with both direct EPR and spin trapping technique. | ||
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- | **Stimulated Luminescence instrumentation** | + | ===== Stimulated Luminescence instrumentation ===== |
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=== Thermoluminescence reader === | === Thermoluminescence reader === | ||
- | {{aree:epr:altre_risorse:lettore_termoluminescenza_harshaw3500-num.jpeg?300|Thermoluminescence reader + laboratory oven for thermal treatments}} {{:aree:epr:altre_risorse:lettore_termoluminescenza_harshaw3500-dett.jpeg?300|Detail of thermoluminescence reader}} | + | |
- | \\ \\ | + | {{aree:epr:altre_risorse:lettore_termoluminescenza_harshaw3500-num.jpeg?300 |Thermoluminescence reader + laboratory oven for thermal treatments}} {{:aree:epr:altre_risorse:lettore_termoluminescenza_harshaw3500-dett.jpeg?300 |Detail of thermoluminescence reader}} Thermoluminescence reader Harshaw, mod. 3500 equipped with software WinREMS for data acquisition and analysis. Thermal cycles up to 600°C (with an uncertainty of +/-1%) with different heating rates, including isothermal decays. Laboratory oven specially designed for TL dosimetry. Run by a microprocessor, it allows heating of the sample at controlled temperatures. Different heating cycles available by the dedicated software. |
- | \\ Thermoluminescence reader Harshaw, mod. 3500 equipped with software WinREMS for data acquisition and analysis. Thermal cycles up to 600°C (with an uncertainty of +/-1%) with different heating rates, including isothermal decays. Laboratory oven specially designed for TL dosimetry. Run by a microprocessor, it allows heating of the sample at controlled temperatures. Different heating cycles available by the dedicated software. | + | |
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=== Optically stimulated luminescence reader === | === Optically stimulated luminescence reader === | ||
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{{:aree:epr:altre_risorse:lettore_osl-tl_riso_da-20.jpeg?300 |OSL/TL reader}} OSL/TL reader Risø DA-20 (DTU Nutech, Danimarca) equipped with a carousel loading up to 48 samples placed on steel plates of about 10mm in diameter and 0.25mm in thickness. Managed by a dedicated software that allows to set OSL and TL measurements in sequence. The optical stimulation system consists of infrared and visible LEDs; currently it allows stimulation with blue light (470 ± 30nm, with a maximum power of 36mW/cm² in the sample position). The heating system for thermal stimulation allows reaching the maximum temperature of 700°C. Different optical filters are available to select the frequency range of the emitted luminescence. Housing for a radioactive source (<sup>90</sup>Sr/<sup>90</sup>Y or <sup>241</sup>Am) available for sample calibration. | {{:aree:epr:altre_risorse:lettore_osl-tl_riso_da-20.jpeg?300 |OSL/TL reader}} OSL/TL reader Risø DA-20 (DTU Nutech, Danimarca) equipped with a carousel loading up to 48 samples placed on steel plates of about 10mm in diameter and 0.25mm in thickness. Managed by a dedicated software that allows to set OSL and TL measurements in sequence. The optical stimulation system consists of infrared and visible LEDs; currently it allows stimulation with blue light (470 ± 30nm, with a maximum power of 36mW/cm² in the sample position). The heating system for thermal stimulation allows reaching the maximum temperature of 700°C. Different optical filters are available to select the frequency range of the emitted luminescence. Housing for a radioactive source (<sup>90</sup>Sr/<sup>90</sup>Y or <sup>241</sup>Am) available for sample calibration. | ||
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=== Pulsed Photostimulated Luminescence (PPSL) === | === Pulsed Photostimulated Luminescence (PPSL) === | ||
- | {{aree:epr:altre_risorse:lettore_ppsl_suerc.jpeg?250|}} {{aree:epr:altre_risorse:lettore_ppsl_suerc-dett.jpeg?250|}} | ||
- | Made by the Scottish Universities Environmental Research Centre (SUERC, Glasgow Scotland) for analysis on irradiated foods. The instrument can be connected to a computer and managed by appropriate software. Equipped with a stimulation system of infrared LEDs. Stimulation occurs by pulses: the duration of the single pulse and the number of pulses can be adjusted by the instrument management software. | ||
- | \\<wrap tip>**[[en:aree:epr:altre_risorse:luminescenze|About the phenomena of Stimulated Luminescence]]**</wrap> | + | {{aree:epr:altre_risorse:lettore_ppsl_suerc.jpeg?250 |}} {{aree:epr:altre_risorse:lettore_ppsl_suerc-dett.jpeg?250 |}} Made by the Scottish Universities Environmental Research Centre (SUERC, Glasgow Scotland) for analysis on irradiated foods. The instrument can be connected to a computer and managed by appropriate software. Equipped with a stimulation system of infrared LEDs. Stimulation occurs by pulses: the duration of the single pulse and the number of pulses can be adjusted by the instrument management software. |
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+ | <wrap tip>**[[en:aree:epr:altre_risorse:luminescenze|About the phenomena of Stimulated Luminescence]]**</wrap> | ||
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> Contacts: | > Contacts: | ||
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> [[sara.dellamonaca@iss.it|Sara Della Monaca]] | > [[sara.dellamonaca@iss.it|Sara Della Monaca]] | ||
> ☎ **+39 06 4990 2483** | > ☎ **+39 06 4990 2483** | ||
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- | **Dosimetric systems** | + | |
+ | =====Dosimetric systems===== | ||
==== Active dosimeters ==== | ==== Active dosimeters ==== | ||
- | {{aree:epr:altre_risorse:dosimetri_attivi-1.png?0x150|}} {{aree:epr:altre_risorse:dosimetri_attivi-2.png?0x150|}} | + | |
+ | {{aree:epr:altre_risorse:dosimetri_attivi-1.png?0x150 |}} {{aree:epr:altre_risorse:dosimetri_attivi-2.png?0x150 |}} | ||
* Markus ionization chamber type N23343 (PTW) | * Markus ionization chamber type N23343 (PTW) | ||
* Silicon diode detector for proton beams type DZA192 (Scanditronix) | * Silicon diode detector for proton beams type DZA192 (Scanditronix) | ||
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* microDiamond detector type 60019 (PTW) | * microDiamond detector type 60019 (PTW) | ||
* Electrometer model 6517A (Keithley) | * Electrometer model 6517A (Keithley) | ||
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==== Passive dosimeters ==== | ==== Passive dosimeters ==== | ||
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=== Alanine/EPR dosimetry system === | === Alanine/EPR dosimetry system === | ||
- | {{:en:aree:epr:altre_risorse:alanine.jpg?300|Alanine/EPR dosimetry system}} | + | |
+ | {{:en:aree:epr:altre_risorse:alanine.jpg?200|Alanine/EPR dosimetry system}} | ||
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=== Thermoluminescence dosimeters (TLD) === | === Thermoluminescence dosimeters (TLD) === | ||
- | {{:en:aree:epr:altre_risorse:dosimetri_termoluminescenza.jpeg?300|Thermoluminescent dosimeters}} | + | |
+ | {{:en:aree:epr:altre_risorse:dosimetri_termoluminescenza.jpeg?200|Thermoluminescent dosimeters}} | ||
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=== Radiochromic film dosimeters === | === Radiochromic film dosimeters === | ||
- | {{:en:aree:epr:altre_risorse:dosimetri_a_film.png?200|}} | + | |
+ | {{:en:aree:epr:altre_risorse:dosimetri_a_film.png?350|}} | ||
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==== Phantoms ==== | ==== Phantoms ==== | ||
- | {{:en:aree:epr:altre_risorse:fantocci.png?300|}} | + | |
+ | {{:en:aree:epr:altre_risorse:fantocci.png?350 |}} | ||
* Alderson Rando phantom | * Alderson Rando phantom | ||
* PMMA phantoms | * PMMA phantoms | ||
* Water phantoms | * Water phantoms | ||
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> Contact: [[cinzia.deangelis@iss.it|Cinzia De Angelis]] | > Contact: [[cinzia.deangelis@iss.it|Cinzia De Angelis]] | ||
> ☎ **+39 06 4990 2248** | > ☎ **+39 06 4990 2248** | ||
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+ | ===== Surface Plasmon Resonance (SPR - Biacore) ===== | ||
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+ | The <wrap em>Surface Plasmon Resonance (SPR)</wrap> technique allows studying the interactions between a ligand and putative substates anchored onto a gold-chip, in a pM range of the bulk, with a very high sensibility (few pg/mm²). **[[.:biacore:|[...read more]]]** | ||
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+ | > Contact: [[donatella.pietraforte@iss.it|Donatella Pietraforte]] | ||
+ | > ☎ **+39 06 4990 2907** | ||
+ | > Where I am: building **20**, floor **D**, room **2s** | ||
/* > FIXME **To be revised.**\\ //(remove this paragraph once is finished)// */ | /* > FIXME **To be revised.**\\ //(remove this paragraph once is finished)// */ |