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en:aree:citometria:strumentazione:analizzatori [2019/06/26 20:18] Gianluca Frustagli Added CytoFLEX LX Flow Cytometer. |
en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:48] (current) Gianluca Frustagli FACScan removed. |
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| ===== CytoFLEX LX - Flow Cytometer ===== | ===== CytoFLEX LX - Flow Cytometer ===== | ||
| - | {{cytoflex_lx.jpeg?300 }} The CytoFLEX LX Flow Cytometer is an expansion of the CytoFLEX Platform. This model expands research possibilities with up to six lasers and 21 color parameters. Six spatially separated lasers allows panels to be spread across the spectrum reducing cross talk and spectral overlap. | + | {{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. |
| - | For higher throughput applications an optional plate loader module is available. | + | Digital signal processing on 7 decades of dynamic range. |
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| + | Excellent resolution of nanoparticles thanks to the new VSSC parameter. | ||
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| + | \\ \\ | ||
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| + | ==== Technical specifications ==== | ||
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| + | * Resolution: | ||
| + | * < 300 nm particles (488 nm side scatter) | ||
| + | * < 80 nm polystyrene particles (405 nm side scatter) | ||
| + | * rCV 3% | ||
| + | * collections vessels: | ||
| + | * 5 ml, 2 ml, 1.5 ml; | ||
| + | * 96w plates; | ||
| + | * absolute counting. | ||
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| - | * {{ :aree:citometria:strumentazione:cytoflex_lx-spec-ita-rev2.pdf |Technical specifications}} | + | ==== Optical configuration ==== |
| - | * {{ :en:aree:citometria:strumentazione:cytoflex_lx-opt_conf.pdf |Optical configuration}} | + | |
| + | 5 activated lasers / 19 color parameters: | ||
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| + | ^ **UV Laser** \\ <wrap lo>355 nm</wrap> ^ **Violet Laser** \\ <wrap lo>405 nm</wrap> ^ **Blue Laser** \\ <wrap lo>488 nm</wrap> ^ **Yellow-Green Laser** \\ <wrap lo>561 nm</wrap> ^ **Red Laser** \\ <wrap lo>640 nm</wrap> ^ | ||
| + | | 405/30 \\ 525/40 \\ 675/30 | 450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/43 | 488/8 SSC \\ 525/40 \\ 610/20 \\ 690/50 | 585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/43 | 660/10 \\ 712/25 \\ 763/43 | | ||
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| ===== FACSCanto - BD Biosciences ===== | ===== FACSCanto - BD Biosciences ===== | ||
| - | {{:aree:citometria:strumentazione:facscanto.png?300 |}} \\ \\ **Technical features (FIXME brochure):** | + | {{:aree:citometria:strumentazione:facscanto.png?300 |}} \\ \\ **Technical features ({{:aree:citometria:strumentazione:facscanto-brochure.pdf|brochure}}):** |
| * 2 laser (6 fluorescence) | * 2 laser (6 fluorescence) | ||
| * 488 nm, 4 PMT | * 488 nm, 4 PMT | ||
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| __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ | __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ | ||
| - | \\ \\ \\ \\ \\ | + | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ |
| - | + | ||
| - | ===== FACScan - BD Biosciences ===== | + | |
| - | {{:aree:citometria:strumentazione:facscan.jpeg?300 |}} **Technical features ({{:aree:citometria:strumentazione:facscan-manual.pdf |manual}}):** | ||
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| - | * 1 laser (3 fluorescence) | ||
| - | * 488 nm, 3 PMT | ||
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| - | \\ \\ \\ \\ \\ \\ \\ \\ | ||
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| - | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ | ||
