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--- en:aree:citometria:strumentazione:analizzatori [2018/02/16 16:34]
Gianluca Frustagli Links fixed
+++ en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:48] (current)
Gianluca Frustagli FACScan removed.
@@ Line 1: / Line 1: @@
-> FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)//
 ~~NOTOC~~
-**Area Citometria**
+**Citometry Area**
+====== Analyzers ======
+===== CytoFLEX LX - Flow Cytometer =====
+{{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously.
+Digital signal processing on 7 decades of dynamic range.
+Excellent resolution of nanoparticles thanks to the new VSSC parameter.
+\\ \\
+==== Technical specifications ====
+  * Resolution:
+    * < 300 nm particles (488 nm side scatter)
+    * < 80 nm polystyrene particles (405 nm side scatter)
+    * rCV 3%
+  * collections vessels:
+    * 5 ml, 2 ml, 1.5 ml;
+    * 96w plates;
+  * absolute counting.
+\\
+==== Optical configuration ====
+activated lasers / 19 color parameters:
+^  **UV Laser** \\ <wrap lo>355 nm</wrap>    ^  **Violet Laser** \\ <wrap lo>405 nm</wrap>       ^  **Blue Laser** \\ <wrap lo>488 nm</wrap>  ^  **Yellow-Green Laser** \\ <wrap lo>561 nm</wrap>  ^  **Red Laser** \\ <wrap lo>640 nm</wrap>  ^
+|  405/30 \\ 525/40 \\ 675/30                |  450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/43   |  488/8 SSC \\ 525/40 \\ 610/20 \\ 690/50   |  585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/43    |  660/10 \\ 712/25 \\ 763/43               |
+\\ \\ \\
-====== Analizzatori ======
 ===== LSR II - BD Biosciences =====
-**Caratteristiche tecniche ({{ :aree:citometria:strumentazione:analizzatore-lsr2-brochure.pdf |brochure}})** {{ :aree:citometria:strumentazione:lsr2.png?300|}}
+**Technical features ({{ :aree:citometria:strumentazione:analizzatore-lsr2-brochure.pdf |brochure}})**: {{ :aree:citometria:strumentazione:lsr2.png?300|}}
-  * 3 laser (8 fluorescenze)
+  * 3 laser (8 fluorescence)
     * 405 nm, 2 PMT
     * 488 nm, 4 PMT
     * 633 nm, 2 PMT
-  * Acquisizione del campione anche da piastre multiwell.
+  * Sample acquisition possible also from multiwell plates.
 \\ \\ \\
 ===== FACSCanto - BD Biosciences =====
-{{:aree:citometria:strumentazione:facscanto.png?300 |}}  \\ \\ **Caratteristiche tecniche (FIXME brochure)**
+{{:aree:citometria:strumentazione:facscanto.png?300 |}}  \\ \\ **Technical features ({{:aree:citometria:strumentazione:facscanto-brochure.pdf|brochure}}):**
-  * 2 laser (6 fluorescenze)
+  * 2 laser (6 fluorescence)
     * 488 nm, 4 PMT
     * 633 nm, 2 PMT
 \\ \\ \\ \\ \\ \\
 ===== GALLIOS - Beckman Coulter =====
-**Caratteristiche tecniche ({{:aree:citometria:strumentazione:gallios-brochure.pdf|brochure}}, {{:aree:citometria:strumentazione:gallios-spec.pdf|specifiche}})** {{ :aree:citometria:strumentazione:gallios.png?300|}}
+**Technical features ({{:aree:citometria:strumentazione:gallios-brochure.pdf|brochure}}, {{:aree:citometria:strumentazione:gallios-spec.pdf|specifications}}):** {{ :aree:citometria:strumentazione:gallios.png?300|}}
-  * 3 laser (10 fluorescenze)
+  * 3 laser (10 fluorescence)
     * 405 nm, 2 PMT
     * 488 nm, 5 PMT
     * 633 nm, 3 PMT
-\\
+__[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__
-**//Norme per gli utenti accreditati al citofluorimetro Gallios//** FIXME da aggiornare
-\\ \\ \\ \\ \\
-===== FACScan - BD Biosciences =====
-{{:aree:citometria:strumentazione:facscan.jpeg?300 |}} **Caratteristiche tecniche ({{:aree:citometria:strumentazione:facscan-manual.pdf |manuale}})**
+/* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */
-  * 1 laser (3 fluorescenze)
-    * 488 nm, 3 PMT