Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision 		Previous revision
Last revision Both sides next revision
en:aree:citometria:strumentazione:analizzatori [2018/08/08 15:03]
Gianluca Frustagli 		en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:37]
Gianluca Frustagli [Technical specifications]
Line 4: Line 4:
  
 ====== Analyzers ====== ====== Analyzers ======
 +
 +===== CytoFLEX LX - Flow Cytometer =====
 +
 +{{:​aree:​citometria:​strumentazione:​cytoflex_lx.jpeg?​300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously.
 +
 +Digital signal processing on 7 decades of dynamic range.
 +
 +Excellent resolution of nanoparticles thanks to the new VSSC parameter.
 +
 +\\ \\
 +
 +==== Technical specifications ====
 +
 +  * Resolution:
 +    * < 300 nm particles (488 nm side scatter)
 +    * < 80 nm polystyrene particles (405 nm side scatter)
 +    * rCV 3%
 +  * collections vessels:
 +    * 5 ml, 2 ml, 1.5 ml;
 +    * 96w plates;
 +  * absolute counting.
 +
 +\\
 +
 +==== Optical configuration ====
 +
 +5 activated lasers / 19 color parameters:
 +
 +^  **UV Laser** \\ <wrap lo>355 nm</​wrap> ​   ^  **Violet Laser** \\ <wrap lo>405 nm</​wrap> ​      ​^ ​ **Blue Laser** \\ <wrap lo>488 nm</​wrap> ​ ^  **Yellow-Green Laser** \\ <wrap lo>561 nm</​wrap> ​ ^  **Red Laser** \\ <wrap lo>640 nm</​wrap> ​ ^
 +|  405/30 \\ 525/40 \\ 675/​30 ​               |  450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/​43 ​  ​| ​ 488/8 SSC \\ 525/40 \\ 610/20 \\ 690/​50 ​  ​| ​ 585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/​43 ​   |  660/10 \\ 712/25 \\ 763/​43 ​              |
 +
 +
 +\\ \\ \\
 +
  
 ===== LSR II - BD Biosciences ===== ===== LSR II - BD Biosciences =====
Line 19: Line 53:
 ===== FACSCanto - BD Biosciences ===== ===== FACSCanto - BD Biosciences =====
  
-{{:​aree:​citometria:​strumentazione:​facscanto.png?​300 |}}  \\ \\ **Technical features (FIXME brochure):​**+{{:​aree:​citometria:​strumentazione:​facscanto.png?​300 |}}  \\ \\ **Technical features ({{:​aree:​citometria:​strumentazione:​facscanto-brochure.pdf|brochure}}):**
   * 2 laser (6 fluorescence)   * 2 laser (6 fluorescence)
     * 488 nm, 4 PMT     * 488 nm, 4 PMT
Line 35: Line 69:
     * 633 nm, 3 PMT     * 633 nm, 3 PMT
  
-\\ +__[[en:​aree:​citometria:​documenti:​pos|Rules for users accredited to the Gallios flow cytometer]]__
-**//Rules for users accredited to the Gallios flow cytometer//** FIXME to update+
  
 \\ \\ \\ \\ \\ \\ \\ \\ \\ \\
Line 48: Line 81:
  
 \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\
-> FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)//+ 
 +/* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// ​*/