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en:aree:citometria:start [2018/08/06 16:24]
Gianluca Frustagli English translation.
en:aree:citometria:start [2023/02/16 19:58] (current)
Gianluca Frustagli
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-> FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// 
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 **Area** **Area**
 ====== Cytometry ====== ====== Cytometry ======
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 The //Core Facility// performs cytometric flow analysis, cytofluorimetric or mass, at the single cell level. The //Core Facility// performs cytometric flow analysis, cytofluorimetric or mass, at the single cell level.
-The Area is equipped with three analyzers that allow analysis of up to 10 fluorescence parameters, two cell sorter capable of separating up to four cell populations on the basis of 10 fluorescence parameters and a mass cytometer (CyTOF) that allows to perform multiparametric analysis ​between 25-35 parameters (up to a theoretical maximum ​of 90), based on the use of antibodies conjugated to rare metals.+The Area is equipped with three analyzers that allow analysis of up to 10 fluorescence parameters, two cell sorter capable of separating up to four cell populations on the basis of 10 fluorescence parameters and a mass cytometer (CyTOF) that allows to perform multiparametric analysis ​upto 35 parameters ​at most (the theoretical maximum ​is 90), based on the use of antibodies conjugated to rare metals ​(Lanthanides)\\
 Cytofluorimetric analysis can be conducted in High-Throughput mode. Cytofluorimetric analysis can be conducted in High-Throughput mode.
  
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 The cytofluorimetry and mass cytometry //Core Facility// aims to promote access to and use of these technologies for ISS researchers and to actively promote both the collaboration and the provision of services to users outside the Institute. The cytofluorimetry and mass cytometry //Core Facility// aims to promote access to and use of these technologies for ISS researchers and to actively promote both the collaboration and the provision of services to users outside the Institute.
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 > Contact: [[massimo.sanchez@iss.it|Massimo Sanchez]] > Contact: [[massimo.sanchez@iss.it|Massimo Sanchez]]
 > ☎ **+39 06 4990 2576-2550** > ☎ **+39 06 4990 2576-2550**
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 +/* > FIXME **To be revised.**\\ //(remove this paragraph once finished)// */