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The ISS sequencing service is equipped with the Ion GeneStudio S5 System, a next-generation sequencing (NGS) system based on semiconductor technology (Ion S5 chip) that enables simple and fast multi-targeted sequencing workflows with high accuracy at reduced costs.
Whenever a single nucleotide is incorporated during DNA polymerization, a hydrogen ion is released: the resulting pH variation is detected by the instrument within each CHIP well. The nucleotide incorporation is associated to each pH variation and the chemical signal is then converted into digital signal. Since the growing complementary DNA strand is flooded with a single species of nucleotide at a time, a signal is detectable only when a nucleotide is successfully incorporated.
The Ion GeneStudio S5 System enables multiplexing of up to 96 fragment libraries on a single sequencing chip thank to the use of specific molecular barcodes for sample-labelling.
For DNA analysis, the required starting material is about 10-100 ng (at 10 ng/µl).
The availability of 4 chip sizes (Ion chip 510, 520, 530 and 540) fits with many different applications and throughput needs and allows to achieve from 2–80 million reads per run.
Ion GeneStudio S5 technology is mainly indicated for the following applications:
Contact: Manuela Marra
☎ +39 06 4990 3051