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                    en:aree:citometria:strumentazione:analizzatori [2019/07/22 15:29] Gianluca Frustagli [CytoFLEX LX - Flow Cytometer] Update.  | 
                
                    en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:48] (current) Gianluca Frustagli FACScan removed.  | 
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| ===== CytoFLEX LX - Flow Cytometer ===== | ===== CytoFLEX LX - Flow Cytometer ===== | ||
| - | {{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. \\ | + | {{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. | 
| - | Digital signal processing on 7 decades of dynamic range. \\ | + | |
| + | Digital signal processing on 7 decades of dynamic range. | ||
| Excellent resolution of nanoparticles thanks to the new VSSC parameter. | Excellent resolution of nanoparticles thanks to the new VSSC parameter. | ||
| - | === Technical specifications === | + | \\ \\ | 
| + | |||
| + | ==== Technical specifications ==== | ||
| * Resolution: | * Resolution: | ||
| - | * < 300 nm particles (488 nm side scattering) | + | * < 300 nm particles (488 nm side scatter) | 
| - | * < 80 nm polystyrene particles (405 nm side scattering) | + | * < 80 nm polystyrene particles (405 nm side scatter) | 
| * rCV 3% | * rCV 3% | ||
| * collections vessels: | * collections vessels: | ||
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| * absolute counting. | * absolute counting. | ||
| + | \\ | ||
| - | === Optical configuration === | + | ==== Optical configuration ==== | 
| 5 activated lasers / 19 color parameters: | 5 activated lasers / 19 color parameters: | ||
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| __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ | __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ | ||
| - | \\ \\ \\ \\ \\ | + | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ | 
| - | ===== FACScan - BD Biosciences ===== | ||
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| - | {{:aree:citometria:strumentazione:facscan.jpeg?300 |}} **Technical features ({{:aree:citometria:strumentazione:facscan-manual.pdf |manual}}):** | ||
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| - | * 1 laser (3 fluorescence) | ||
| - | * 488 nm, 3 PMT | ||
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| - | \\ \\ \\ \\ \\ \\ \\ \\ | ||
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| - | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ | ||