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en:aree:citometria:strumentazione:analizzatori [2019/07/22 15:12] Gianluca Frustagli [CytoFLEX LX - Flow Cytometer] update. |
en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:48] (current) Gianluca Frustagli FACScan removed. |
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| ===== CytoFLEX LX - Flow Cytometer ===== | ===== CytoFLEX LX - Flow Cytometer ===== | ||
| - | {{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. \\ | + | {{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. |
| - | Digital signal processing on 7 decades of dynamic range. \\ | + | |
| + | Digital signal processing on 7 decades of dynamic range. | ||
| Excellent resolution of nanoparticles thanks to the new VSSC parameter. | Excellent resolution of nanoparticles thanks to the new VSSC parameter. | ||
| - | === Technical specifications === | + | \\ \\ |
| + | |||
| + | ==== Technical specifications ==== | ||
| * Resolution: | * Resolution: | ||
| - | * < 300 nm particles (488 nm side scattering) | + | * < 300 nm particles (488 nm side scatter) |
| - | * < 80 nm polystyrene particles (405 nm side scattering) | + | * < 80 nm polystyrene particles (405 nm side scatter) |
| * rCV 3% | * rCV 3% | ||
| * collections vessels: | * collections vessels: | ||
| Line 22: | Line 26: | ||
| * absolute counting. | * absolute counting. | ||
| + | \\ | ||
| - | === Optical configuration === | + | ==== Optical configuration ==== |
| + | 5 activated lasers / 19 color parameters: | ||
| + | |||
| + | ^ **UV Laser** \\ <wrap lo>355 nm</wrap> ^ **Violet Laser** \\ <wrap lo>405 nm</wrap> ^ **Blue Laser** \\ <wrap lo>488 nm</wrap> ^ **Yellow-Green Laser** \\ <wrap lo>561 nm</wrap> ^ **Red Laser** \\ <wrap lo>640 nm</wrap> ^ | ||
| + | | 405/30 \\ 525/40 \\ 675/30 | 450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/43 | 488/8 SSC \\ 525/40 \\ 610/20 \\ 690/50 | 585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/43 | 660/10 \\ 712/25 \\ 763/43 | | ||
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| __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ | __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ | ||
| - | \\ \\ \\ \\ \\ | + | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ |
| - | ===== FACScan - BD Biosciences ===== | ||
| - | |||
| - | {{:aree:citometria:strumentazione:facscan.jpeg?300 |}} **Technical features ({{:aree:citometria:strumentazione:facscan-manual.pdf |manual}}):** | ||
| - | |||
| - | * 1 laser (3 fluorescence) | ||
| - | * 488 nm, 3 PMT | ||
| - | |||
| - | \\ \\ \\ \\ \\ \\ \\ \\ | ||
| - | |||
| - | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ | ||
