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en:aree:citometria:strumentazione:analizzatori [2019/05/16 01:26]
Gianluca Frustagli 		en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:48] (current)
Gianluca Frustagli FACScan removed.
Line 4: Line 4:
  
 ====== Analyzers ====== ====== Analyzers ======
 +
 +===== CytoFLEX LX - Flow Cytometer =====
 +
 +{{:​aree:​citometria:​strumentazione:​cytoflex_lx.jpeg?​300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously.
 +
 +Digital signal processing on 7 decades of dynamic range.
 +
 +Excellent resolution of nanoparticles thanks to the new VSSC parameter.
 +
 +\\ \\
 +
 +==== Technical specifications ====
 +
 +  * Resolution:
 +    * < 300 nm particles (488 nm side scatter)
 +    * < 80 nm polystyrene particles (405 nm side scatter)
 +    * rCV 3%
 +  * collections vessels:
 +    * 5 ml, 2 ml, 1.5 ml;
 +    * 96w plates;
 +  * absolute counting.
 +
 +\\
 +
 +==== Optical configuration ====
 +
 +5 activated lasers / 19 color parameters:
 +
 +^  **UV Laser** \\ <wrap lo>355 nm</​wrap> ​   ^  **Violet Laser** \\ <wrap lo>405 nm</​wrap> ​      ​^ ​ **Blue Laser** \\ <wrap lo>488 nm</​wrap> ​ ^  **Yellow-Green Laser** \\ <wrap lo>561 nm</​wrap> ​ ^  **Red Laser** \\ <wrap lo>640 nm</​wrap> ​ ^
 +|  405/30 \\ 525/40 \\ 675/​30 ​               |  450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/​43 ​  ​| ​ 488/8 SSC \\ 525/40 \\ 610/20 \\ 690/​50 ​  ​| ​ 585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/​43 ​   |  660/10 \\ 712/25 \\ 763/​43 ​              |
 +
 +
 +\\ \\ \\
 +
  
 ===== LSR II - BD Biosciences ===== ===== LSR II - BD Biosciences =====
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 ===== FACSCanto - BD Biosciences ===== ===== FACSCanto - BD Biosciences =====
  
-{{:​aree:​citometria:​strumentazione:​facscanto.png?​300 |}}  \\ \\ **Technical features (FIXME brochure):​**+{{:​aree:​citometria:​strumentazione:​facscanto.png?​300 |}}  \\ \\ **Technical features ({{:​aree:​citometria:​strumentazione:​facscanto-brochure.pdf|brochure}}):**
   * 2 laser (6 fluorescence)   * 2 laser (6 fluorescence)
     * 488 nm, 4 PMT     * 488 nm, 4 PMT
Line 35: Line 69:
     * 633 nm, 3 PMT     * 633 nm, 3 PMT
  
-[[en:aree:​citometria:​documenti:​pos|Norme per gli utenti accreditati al citofluorimetro ​Gallios]]+__[[en:​aree:​citometria:​documenti:​pos|Rules for users accredited to the Gallios ​flow cytometer]]__
  
-\\ \\ \\ \\ \\ +/* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */
- +
-===== FACScan - BD Biosciences ===== +
- +
-{{:​aree:​citometria:​strumentazione:​facscan.jpeg?300 |}} **Technical features ​({{:​aree:​citometria:​strumentazione:​facscan-manual.pdf |manual}}):**+
  
-  * 1 laser (3 fluorescence) 
-    * 488 nm, 3 PMT 
- 
-\\ \\ \\ \\ \\ \\ \\ \\ 
- 
-/* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */