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en:aree:citometria:strumentazione:analizzatori [2018/02/16 16:02]
Gianluca Frustagli created 		en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:48] (current)
Gianluca Frustagli FACScan removed.
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-> FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)// 
- 
 ~~NOTOC~~ ~~NOTOC~~
  
-**Area ​Citometria**+**Citometry ​Area** 
 + 
 +====== Analyzers ====== 
 + 
 +===== CytoFLEX LX - Flow Cytometer ===== 
 + 
 +{{:​aree:​citometria:​strumentazione:​cytoflex_lx.jpeg?​300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. 
 + 
 +Digital signal processing on 7 decades of dynamic range. 
 + 
 +Excellent resolution of nanoparticles thanks to the new VSSC parameter. 
 + 
 +\\ \\ 
 + 
 +==== Technical specifications ==== 
 + 
 +  * Resolution:​ 
 +    * < 300 nm particles (488 nm side scatter) 
 +    * < 80 nm polystyrene particles (405 nm side scatter) 
 +    * rCV 3% 
 +  * collections vessels: 
 +    * 5 ml, 2 ml, 1.5 ml; 
 +    * 96w plates; 
 +  * absolute counting. 
 + 
 +\\ 
 + 
 +==== Optical configuration ==== 
 + 
 +5 activated lasers / 19 color parameters:​ 
 + 
 +^  **UV Laser** \\ <wrap lo>355 nm</​wrap> ​   ^  **Violet Laser** \\ <wrap lo>405 nm</​wrap> ​      ​^ ​ **Blue Laser** \\ <wrap lo>488 nm</​wrap> ​ ^  **Yellow-Green Laser** \\ <wrap lo>561 nm</​wrap> ​ ^  **Red Laser** \\ <wrap lo>640 nm</​wrap> ​ ^ 
 +|  405/30 \\ 525/40 \\ 675/​30 ​               |  450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/​43 ​  ​| ​ 488/8 SSC \\ 525/40 \\ 610/20 \\ 690/​50 ​  ​| ​ 585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/​43 ​   |  660/10 \\ 712/25 \\ 763/​43 ​              | 
 + 
 + 
 +\\ \\ \\
  
-====== Analizzatori ====== 
  
 ===== LSR II - BD Biosciences ===== ===== LSR II - BD Biosciences =====
  
-**Caratteristiche tecniche ​({{ analizzatore-lsr2-brochure.pdf |brochure}})** {{ lsr2.png?​300|}}+**Technical features ​({{ :​aree:​citometria:​strumentazione:​analizzatore-lsr2-brochure.pdf |brochure}})**: {{ :​aree:​citometria:​strumentazione:​lsr2.png?​300|}}
  
-  * 3 laser (8 fluorescenze)+  * 3 laser (8 fluorescence)
     * 405 nm, 2 PMT     * 405 nm, 2 PMT
     * 488 nm, 4 PMT     * 488 nm, 4 PMT
     * 633 nm, 2 PMT     * 633 nm, 2 PMT
-  * Acquisizione del campione anche da piastre ​multiwell.+  * Sample acquisition possible also from multiwell ​plates.
  
 \\ \\ \\ \\ \\ \\
 +
 ===== FACSCanto - BD Biosciences ===== ===== FACSCanto - BD Biosciences =====
  
-{{facscanto.png?​300 |}}  \\ \\ **Caratteristiche tecniche ​(FIXME brochure)** +{{:​aree:​citometria:​strumentazione:​facscanto.png?​300 |}}  \\ \\ **Technical features ​({{:​aree:​citometria:​strumentazione:​facscanto-brochure.pdf|brochure}}):** 
-  * 2 laser (6 fluorescenze)+  * 2 laser (6 fluorescence)
     * 488 nm, 4 PMT     * 488 nm, 4 PMT
     * 633 nm, 2 PMT     * 633 nm, 2 PMT
  
 \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\
 +
 ===== GALLIOS - Beckman Coulter ===== ===== GALLIOS - Beckman Coulter =====
  
-**Caratteristiche tecniche ​({{gallios-brochure.pdf|brochure}},​ {{gallios-spec.pdf|specifiche}})** {{ gallios.png?​300|}}+**Technical features ​({{:​aree:​citometria:​strumentazione:​gallios-brochure.pdf|brochure}},​ {{:​aree:​citometria:​strumentazione:​gallios-spec.pdf|specifications}}):** {{ :​aree:​citometria:​strumentazione:​gallios.png?​300|}}
  
-  * 3 laser (10 fluorescenze)+  * 3 laser (10 fluorescence)
     * 405 nm, 2 PMT     * 405 nm, 2 PMT
     * 488 nm, 5 PMT     * 488 nm, 5 PMT
     * 633 nm, 3 PMT     * 633 nm, 3 PMT
  
-\\ +__[[en:​aree:​citometria:​documenti:​pos|Rules for users accredited to the Gallios ​flow cytometer]]__
-**//Norme per gli utenti accreditati al citofluorimetro ​Gallios//** FIXME da aggiornare +
- +
-\\ \\ \\ \\ \\ +
-===== FACScan - BD Biosciences =====+
  
-{{:​aree:​citometria:​strumentazione:​facscan.jpeg?300 |}} **Caratteristiche tecniche ​({{facscan-manual.pdf |manuale}})**+/* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */
  
-  * 1 laser (3 fluorescenze) 
-    * 488 nm, 3 PMT