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en:aree:citometria:strumentazione:analizzatori [2018/08/06 19:32] Gianluca Frustagli |
en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:48] (current) Gianluca Frustagli FACScan removed. |
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- | > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// | ||
- | |||
~~NOTOC~~ | ~~NOTOC~~ | ||
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====== Analyzers ====== | ====== Analyzers ====== | ||
+ | |||
+ | ===== CytoFLEX LX - Flow Cytometer ===== | ||
+ | |||
+ | {{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. | ||
+ | |||
+ | Digital signal processing on 7 decades of dynamic range. | ||
+ | |||
+ | Excellent resolution of nanoparticles thanks to the new VSSC parameter. | ||
+ | |||
+ | \\ \\ | ||
+ | |||
+ | ==== Technical specifications ==== | ||
+ | |||
+ | * Resolution: | ||
+ | * < 300 nm particles (488 nm side scatter) | ||
+ | * < 80 nm polystyrene particles (405 nm side scatter) | ||
+ | * rCV 3% | ||
+ | * collections vessels: | ||
+ | * 5 ml, 2 ml, 1.5 ml; | ||
+ | * 96w plates; | ||
+ | * absolute counting. | ||
+ | |||
+ | \\ | ||
+ | |||
+ | ==== Optical configuration ==== | ||
+ | |||
+ | 5 activated lasers / 19 color parameters: | ||
+ | |||
+ | ^ **UV Laser** \\ <wrap lo>355 nm</wrap> ^ **Violet Laser** \\ <wrap lo>405 nm</wrap> ^ **Blue Laser** \\ <wrap lo>488 nm</wrap> ^ **Yellow-Green Laser** \\ <wrap lo>561 nm</wrap> ^ **Red Laser** \\ <wrap lo>640 nm</wrap> ^ | ||
+ | | 405/30 \\ 525/40 \\ 675/30 | 450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/43 | 488/8 SSC \\ 525/40 \\ 610/20 \\ 690/50 | 585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/43 | 660/10 \\ 712/25 \\ 763/43 | | ||
+ | |||
+ | |||
+ | \\ \\ \\ | ||
+ | |||
===== LSR II - BD Biosciences ===== | ===== LSR II - BD Biosciences ===== | ||
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===== FACSCanto - BD Biosciences ===== | ===== FACSCanto - BD Biosciences ===== | ||
- | {{:aree:citometria:strumentazione:facscanto.png?300 |}} \\ \\ **Technical features (FIXME brochure):** | + | {{:aree:citometria:strumentazione:facscanto.png?300 |}} \\ \\ **Technical features ({{:aree:citometria:strumentazione:facscanto-brochure.pdf|brochure}}):** |
* 2 laser (6 fluorescence) | * 2 laser (6 fluorescence) | ||
* 488 nm, 4 PMT | * 488 nm, 4 PMT | ||
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* 633 nm, 3 PMT | * 633 nm, 3 PMT | ||
- | \\ | + | __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ |
- | **//Rules for users accredited to the Gallios flow cytometer//** FIXME to update | + | |
- | + | ||
- | \\ \\ \\ \\ \\ | + | |
- | + | ||
- | ===== FACScan - BD Biosciences ===== | + | |
- | {{:aree:citometria:strumentazione:facscan.jpeg?300 |}} **Technical features ({{:aree:citometria:strumentazione:facscan-manual.pdf |manual}}):** | + | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ |
- | * 1 laser (3 fluorescence) | ||
- | * 488 nm, 3 PMT |