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en:aree:citometria:strumentazione:analizzatori [2018/02/16 16:34] Gianluca Frustagli Links fixed |
en:aree:citometria:strumentazione:analizzatori [2019/07/31 14:37] Gianluca Frustagli [Technical specifications] |
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- | > FIXME **This page is not fully translated, yet.**\\ //(remove this paragraph once the translation is finished)// | ||
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~~NOTOC~~ | ~~NOTOC~~ | ||
- | **Area Citometria** | + | **Citometry Area** |
+ | |||
+ | ====== Analyzers ====== | ||
+ | |||
+ | ===== CytoFLEX LX - Flow Cytometer ===== | ||
+ | |||
+ | {{:aree:citometria:strumentazione:cytoflex_lx.jpeg?300 }} The CytoFLEX LX flow cytometer is an expansion of the CytoFLEX platform: a unique instrument based on photodiode technology, equipped with 5 lasers, capable of detecting up to 19 fluorescences and 2 scattering parameters simultaneously. | ||
+ | |||
+ | Digital signal processing on 7 decades of dynamic range. | ||
+ | |||
+ | Excellent resolution of nanoparticles thanks to the new VSSC parameter. | ||
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+ | \\ \\ | ||
+ | |||
+ | ==== Technical specifications ==== | ||
+ | |||
+ | * Resolution: | ||
+ | * < 300 nm particles (488 nm side scatter) | ||
+ | * < 80 nm polystyrene particles (405 nm side scatter) | ||
+ | * rCV 3% | ||
+ | * collections vessels: | ||
+ | * 5 ml, 2 ml, 1.5 ml; | ||
+ | * 96w plates; | ||
+ | * absolute counting. | ||
+ | |||
+ | \\ | ||
+ | |||
+ | ==== Optical configuration ==== | ||
+ | |||
+ | 5 activated lasers / 19 color parameters: | ||
+ | |||
+ | ^ **UV Laser** \\ <wrap lo>355 nm</wrap> ^ **Violet Laser** \\ <wrap lo>405 nm</wrap> ^ **Blue Laser** \\ <wrap lo>488 nm</wrap> ^ **Yellow-Green Laser** \\ <wrap lo>561 nm</wrap> ^ **Red Laser** \\ <wrap lo>640 nm</wrap> ^ | ||
+ | | 405/30 \\ 525/40 \\ 675/30 | 450/45 \\ 525/40 \\ 610/20 \\ 660/10 \\ 763/43 | 488/8 SSC \\ 525/40 \\ 610/20 \\ 690/50 | 585/42 \\ 610/20 \\ 675/30 \\ 710/50 \\ 763/43 | 660/10 \\ 712/25 \\ 763/43 | | ||
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+ | |||
+ | \\ \\ \\ | ||
- | ====== Analizzatori ====== | ||
===== LSR II - BD Biosciences ===== | ===== LSR II - BD Biosciences ===== | ||
- | **Caratteristiche tecniche ({{ :aree:citometria:strumentazione:analizzatore-lsr2-brochure.pdf |brochure}})** {{ :aree:citometria:strumentazione:lsr2.png?300|}} | + | **Technical features ({{ :aree:citometria:strumentazione:analizzatore-lsr2-brochure.pdf |brochure}})**: {{ :aree:citometria:strumentazione:lsr2.png?300|}} |
- | * 3 laser (8 fluorescenze) | + | * 3 laser (8 fluorescence) |
* 405 nm, 2 PMT | * 405 nm, 2 PMT | ||
* 488 nm, 4 PMT | * 488 nm, 4 PMT | ||
* 633 nm, 2 PMT | * 633 nm, 2 PMT | ||
- | * Acquisizione del campione anche da piastre multiwell. | + | * Sample acquisition possible also from multiwell plates. |
\\ \\ \\ | \\ \\ \\ | ||
+ | |||
===== FACSCanto - BD Biosciences ===== | ===== FACSCanto - BD Biosciences ===== | ||
- | {{:aree:citometria:strumentazione:facscanto.png?300 |}} \\ \\ **Caratteristiche tecniche (FIXME brochure)** | + | {{:aree:citometria:strumentazione:facscanto.png?300 |}} \\ \\ **Technical features ({{:aree:citometria:strumentazione:facscanto-brochure.pdf|brochure}}):** |
- | * 2 laser (6 fluorescenze) | + | * 2 laser (6 fluorescence) |
* 488 nm, 4 PMT | * 488 nm, 4 PMT | ||
* 633 nm, 2 PMT | * 633 nm, 2 PMT | ||
\\ \\ \\ \\ \\ \\ | \\ \\ \\ \\ \\ \\ | ||
+ | |||
===== GALLIOS - Beckman Coulter ===== | ===== GALLIOS - Beckman Coulter ===== | ||
- | **Caratteristiche tecniche ({{:aree:citometria:strumentazione:gallios-brochure.pdf|brochure}}, {{:aree:citometria:strumentazione:gallios-spec.pdf|specifiche}})** {{ :aree:citometria:strumentazione:gallios.png?300|}} | + | **Technical features ({{:aree:citometria:strumentazione:gallios-brochure.pdf|brochure}}, {{:aree:citometria:strumentazione:gallios-spec.pdf|specifications}}):** {{ :aree:citometria:strumentazione:gallios.png?300|}} |
- | * 3 laser (10 fluorescenze) | + | * 3 laser (10 fluorescence) |
* 405 nm, 2 PMT | * 405 nm, 2 PMT | ||
* 488 nm, 5 PMT | * 488 nm, 5 PMT | ||
* 633 nm, 3 PMT | * 633 nm, 3 PMT | ||
- | \\ | + | __[[en:aree:citometria:documenti:pos|Rules for users accredited to the Gallios flow cytometer]]__ |
- | **//Norme per gli utenti accreditati al citofluorimetro Gallios//** FIXME da aggiornare | + | |
\\ \\ \\ \\ \\ | \\ \\ \\ \\ \\ | ||
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===== FACScan - BD Biosciences ===== | ===== FACScan - BD Biosciences ===== | ||
- | {{:aree:citometria:strumentazione:facscan.jpeg?300 |}} **Caratteristiche tecniche ({{:aree:citometria:strumentazione:facscan-manual.pdf |manuale}})** | + | {{:aree:citometria:strumentazione:facscan.jpeg?300 |}} **Technical features ({{:aree:citometria:strumentazione:facscan-manual.pdf |manual}}):** |
- | * 1 laser (3 fluorescenze) | + | * 1 laser (3 fluorescence) |
* 488 nm, 3 PMT | * 488 nm, 3 PMT | ||
+ | |||
+ | \\ \\ \\ \\ \\ \\ \\ \\ | ||
+ | |||
+ | /* > FIXME **To be revised.**\\ //(remove this paragraph once the translation is finished)// */ | ||
+ |